Team:Paris/Transduction overview strategy
From 2009.igem.org
Christophe.R (Talk | contribs) (→C.x Fec) |
Christophe.R (Talk | contribs) (→Overview) |
||
Line 5: | Line 5: | ||
== Overview == | == Overview == | ||
* [[Team:Paris/Transduction_overview#Overview | Introduction]] | * [[Team:Paris/Transduction_overview#Overview | Introduction]] | ||
- | * [[Team:Paris/Transduction_overview_fusion |A. Fusion ]] | + | * [[Team:Paris/Transduction_overview_fusion#bottom |A. Fusion ]] |
- | ** [[Team:Paris/Transduction_overview_fusion# | + | ** [[Team:Paris/Transduction_overview_fusion#A.1 Jun/Fos|A.1 jun/fos]] |
- | ** [[Team:Paris/Transduction_overview_fusion# | + | ** [[Team:Paris/Transduction_overview_fusion#A.2 G3P|A.2 G3P]] |
- | ** [[Team:Paris/Transduction_overview_fusion# | + | ** [[Team:Paris/Transduction_overview_fusion#A.3 Snares |A.3 Snares]] |
- | * [[Team:Paris/Transduction_overview_transduction |B. Transduction]] | + | * [[Team:Paris/Transduction_overview_transduction#bottom |B. Transduction]] |
- | ** [[Team:Paris/Transduction_overview_transduction# | + | ** [[Team:Paris/Transduction_overview_transduction#B.1 ABC transporters|B.1 ABC transporters]] |
- | ** [[Team:Paris/Transduction_overview_transduction# | + | ** [[Team:Paris/Transduction_overview_transduction#B.2 Two-component system|B.2 Two Component System]] |
* [[Team:Paris/Transduction_overview_strategy#bottom |C. Our strategy]] | * [[Team:Paris/Transduction_overview_strategy#bottom |C. Our strategy]] | ||
* [[Team:Paris/Transduction_overview_construction#bottom |D. Construction]] | * [[Team:Paris/Transduction_overview_construction#bottom |D. Construction]] |
Revision as of 20:15, 2 September 2009
iGEM > Paris > Reception > Our strategy
C. Our Strategy
C.x Fec
Few ABC transporter such as FecABCD (iron transporter) are able to induce a response regardless of the tranlocation, due to the activity of FecA, some mutant can also have a constitutive expression of FecABCD . We could use FecA- mutant receiver and FecA+ mutant donor to transfert working FecA to the receiver. In this case the receiver will express the FecABCD operon, and so we could place under the control of the Fec ABCD promoter the gene sequence encoding for the response.
problems : the message is unidirectionnal and unrepeatable. It would just be a proof of principle that a vesicle-mediated controlled communication is possible.
C.x The trick TCS
We could with the help of Alfonso Jaramillo design a synthetic PBP which could detect the substrate we choosed and activate a specific HK. The previous work (Valencia 2006) design a vanilin-sensitive PBP and a network for a graduated response whereas we just need a proteic sensitive PBP and a binary type of response.
strain and part needed: strain : E.coli MRi7 (unable to translocate ribose from periplasm to cytoplasme)
part :