Team:Paris/Transduction testing Trans

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iGEM > Paris > Reception > Testing > Transduction

WetLab

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Reception


Transduction

Transduction : the construction of the system that will be able de decypher the message.


the transduction system of the fec operon (FecA, FecR, FecI).


plasmid =


plasmid =



Time required : A lOT !!!!!



Experiments ran :

row 1 row 2 row 3
PCR :



PCR : PCR :


Verification on gel :

ok

Verification on gel :

ok

Verification on gel :

ok

Purification on gel :

ok

Purification on gel :

ok

Purification on gel :

ok


digestion:



digestion: digestion:
verification digestion:

ok

verification digestion:

ok

verification digestion:

ok


ligation:


ligation: ligation:


PCR colo :

ok

PCR colo :

ok

PCR colo :

ok


miniprep:

clone :

miniprep:

clone :

miniprep:

clone :


sequencing :

ok

sequencing :

ok

sequencing :

ok