Team:Paris/constructions

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 +
<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/constructions#bottom | Constructions in progress]]
{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
-
==Freezer==
 
-
<center>
+
== Constructions ==
-
[[Team:Paris/Freezer_Primers#Freezer | Primers]] -
+
<html>
-
[[Team:Paris/Freezer_PCR_products#Freezer | PCR products]] - 
+
<style type="text/css">
-
[[Team:Paris/Freezer_Plasmids#Freezer | Plasmids]] -
+
#left-side {
-
[[Team:Paris/Freezer_Digestion_Products#Freezer | Digestion Products]] -
+
    position: absolute;
-
[[Team:Paris/Freezer_Unexpected_digestion_results#Freezer | Unexpected digestion results]] -
+
    height: 23px;
-
[[Team:Paris/Freezer_Strains_(Glycerol stock)#Freezer | Strains (Glycerol stock)]] - [[Team:Paris/constructions#Freezer | Construction in progress]]
+
    width: 30px;
 +
    top: 0px;
 +
    left: 10px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
 +
    z-index:4;
 +
}
 +
 
 +
#middle-side {
 +
    height: 25px;
 +
    width: 660px;
 +
    position: absolute;
 +
    top: 0px;
 +
    left: 30px;
 +
    margin-top:10px;
 +
    padding-top: 5px;
 +
    background: #dadada;
 +
    z-index:5;
 +
}
 +
 
 +
#right-side {
 +
    position: absolute;
 +
    height: 23px;
 +
    width: 30px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    top: 0px;
 +
    left: 670px;
 +
    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
 +
    z-index:4;
 +
}
 +
 
 +
a.menu_sub {
 +
    padding-left: 7px;
 +
    padding-right: 7px;
 +
}
 +
 
 +
a.menu_sub_active {
 +
    padding-left: 7px;
 +
    padding-right: 7px;
 +
    color:#b0310e;
 +
    font-weight:bold;
 +
}
 +
</style>
 +
<div id="left-side"></div>
 +
<div id="middle-side"><center>
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
 +
<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
 +
<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
</center>
</center>
-
<br>
+
</div>
 +
 
 +
<div id="right-side"></div>
 +
 
 +
</html>
 +
 
 +
 
 +
 
{|
{|
|- style="background: #c5c5c5;"
|- style="background: #c5c5c5;"
Line 26: Line 85:
|width=10% style="background: #FF0066;"| Chloranphenicol
|width=10% style="background: #FF0066;"| Chloranphenicol
|}
|}
-
<html>
 
-
</div>
 
-
<div id="paris_content_boxtop">
 
-
</div>
 
-
<div id="paris_content">
 
-
</html>
 
-
== Constructions ==
+
 
 +
*'''Plasmid A'''
 +
 
 +
 
{|
{|
|- style="background: #0d3e99; text-align: center; color:white;"
|- style="background: #0d3e99; text-align: center; color:white;"
Line 43: Line 99:
|width=4%| Registry
|width=4%| Registry
-
|- style="background: #ccccff; text-align: center"
+
|- style="background: #FF9933; text-align: center"
-
|GC
+
|CC
-
|((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3
+
|(Pbad :: rbs - lacI - LVA) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]???Pbad is on PSB2K3
-
|BBa_blabla :: BBa_tralala in truc
+
|[http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09)
+
|pSB1A3 transformed, digested, PBad transformed and not digested well- LacI transformed and digested (10/08/09)
-
|12
+
|??
-
|BBa_pouet pouet
+
|BBa_????
-
|- style="background: #ccff00; text-align: center"
+
|- style="background: #FF9933; text-align: center"
|CC
|CC
-
|Plac :: rbs-tetR in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|(Pbad :: rbs - lacI - LVA) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
|[http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|
+
|ter transformed, digested and purified (10/08/09)
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #ccff00; text-align: center"
+
|- style="background: #FF9933; text-align: center"
|CC
|CC
-
|(Plac :: rbs-tetR) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|((Pbad :: rbs - lacI - LVA) :: RFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
|BBa_???? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 BBa_K093005] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|
+
|pSB2K3 transformed and digested, RFP : primers designed but not yet ordered (10/08/09)
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #ccff00; text-align: center"
+
 
 +
|- style="background: #FF9933; text-align: center"
|CC
|CC
-
|ter :: ((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|(((Pbad :: rbs - lacI - LVA) :: RFP) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
|[http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
|
|
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #00FF00; text-align: center"
+
 
 +
|- style="background: #FFCC00; text-align: center"
|CC
|CC
-
|Ptet :: (rbs - OmpAsignal :: TolRII) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|(OmpA Linker :: g3p) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|[http://partsregistry.org/Part:BBa_K103006 BBa_K103006] :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|OmpA linker standardisé - OmpA amplified, not purified - Same for g3p (10/08/09)
 +
|??
 +
|BBa_????
 +
 
 +
|- style="background: #FFCC00; text-align: center"
|
|
 +
|(g3p :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|autre possibilité (souf)- 1 primer missing for C term fusion with clyA (10/08/09)
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #00FF00; text-align: center"
+
|- style="background: #FFCC00; text-align: center"
-
|CC
+
|CC!
-
|rbs :: EYFP in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|((OmpA Linker :: g3p) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
| BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
|
|
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #00FF00; text-align: center"
+
|- style="background: #FFCC00; text-align: center"
-
|CC
+
|CC!
-
|Ptet :: (rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|((???autotransporter??? - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
| BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|jun/foss has to be synthetized & autotransporter to be found!!!! (10/08/09)
 +
|??
 +
|BBa_????
 +
 
 +
|- style="background: #FF0000; text-align: center"
 +
|CC!
 +
|((rbs - fecA) :: ((rbs - jun/foss) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|FecA : oligo ordered but not received yet (10/08/09)
 +
|??
 +
|BBa_????
 +
 
 +
|- style="background: #FF0000; text-align: center"
 +
|CC!
 +
|((rbs - fecA) :: ((Ompa Linker :: g3p) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
|
|
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #33CC00; text-align: center"
+
|- style="background: #FF0000; text-align: center"
|CC
|CC
-
|(Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|((rbs - ClyA :: RFP) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|
+
|modif RFP (souf)- some troubles with amplification of clyA fusion C term (10/08/09)
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #33CC00; text-align: center"
+
|- style="background: #FF0000; text-align: center"
|CC
|CC
-
|(Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|((rbs-RFP :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|
+
|modif RFP (souf) - fusion N term de cly A (autre possibilité)- we still miss 1 primer for clyA N term fusion (10/08/09)
 +
|??
 +
|BBa_????
 +
 
 +
|- style="background: #FF0000; text-align: center"
 +
|CC!
 +
|((rbs - ClyA :: RFP)) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|modif RFP (souf)
 +
|??
 +
|BBa_????
 +
 
 +
|- style="background: #FF0000; text-align: center"
 +
|CC!
 +
|((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|modif RFP (souf)
|??
|??
|BBa_????
|BBa_????
|- style="background: #CC0000; text-align: center"
|- style="background: #CC0000; text-align: center"
-
|CC
+
|CC!
-
|Pbad :: rbs - lacI - LVA in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|(Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|[http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|[http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
|
|
|??
|??
Line 124: Line 222:
|- style="background: #CC0000; text-align: center"
|- style="background: #CC0000; text-align: center"
-
|CC
+
|CC!
-
|(Pbad :: rbs - lacI - LVA) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|(Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
|
|
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #CC0000; text-align: center"
+
|- style="background: #990000; text-align: center; color:white;"
-
|CC
+
|CC!
-
|(Pbad :: rbs - lacI - LVA) :: RFP in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 BBa_K093005] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|2*Pbad et 2*ter?? (souf)
 +
|??
 +
|BBa_????
 +
 
 +
|- style="background: #990000; text-align: center; color:white;"
 +
|CC!
 +
|((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
|
|
|??
|??
Line 140: Line 246:
-
|- style="background: #CC0000; text-align: center"
+
|}
 +
 
 +
 
 +
*'''Plasmid B'''
 +
 
 +
 
 +
{|
 +
|- style="background: #0d3e99; text-align: center; color:white;"
 +
|width=3%| Designer
 +
|width=40%| Parts description
 +
|width=25%| Corresponding BioBricks
 +
|width=25%| Status (last update)
 +
|width=3%| Plasmid #
 +
|width=4%| Registry
 +
 
 +
|- style="background: #ccccff; text-align: center"
 +
|GC <span/ id="ptet">
 +
|((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3
 +
|BBa_blabla :: BBa_tralala in truc
 +
|Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09)
 +
|12
 +
|BBa_pouet pouet
 +
 
 +
|- style="background: #ccff00; text-align: center"
|CC
|CC
-
|((Pbad :: rbs - lacI - LVA) :: RFP) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+
|(Plac :: rbs-tetR) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
|BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|[http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
|
+
|both have been digested and purified, ligation in progress (10/08/09)
|??
|??
|BBa_????
|BBa_????
 +
|- style="background: #ccff00; text-align: center"
 +
|CC
 +
|((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|BBa_???? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|
 +
|??
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015]
-
|- style="background: #FF9933; text-align: center"
+
|- style="background: #ccff00; text-align: center"
|CC
|CC
-
|OmpA Linker :: g3p in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|(ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
|[http://partsregistry.org/Part:BBa_K103006 BBa_K103006] :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|[http://partsregistry.org/Part:BBa_B0014 BBa_B0014] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|
|
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #FF9933; text-align: center"
+
|- style="background: #00FF00; text-align: center"
 +
|CL
 +
|(rbs - OmpAsignal :: TolR II)
 +
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|OmpA signal and TolRII amplified, but neither digested nor purified (10/08/09)
 +
|??
 +
|BBa_????
 +
 
 +
|- style="background: #00FF00; text-align: center"
 +
|CC <span/ id="tolr">
 +
|(Ptet :: (rbs - OmpAsignal :: TolRII)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|Ptet digested and purified (10/08/09)
 +
|??
 +
|BBa_????
 +
 
 +
|- style="background: #00FF00; text-align: center"
|CC
|CC
-
|(OmpA Linker :: g3p) :: ter in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|(rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
| BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
|
+
|y a t-il vraiment 2 biobricks ou bien met-on le RBS direct dans des oligos pour RFP? (souf) biobrick to find or primers to design (10/08/09)
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #FF9933; text-align: center"
+
|- style="background: #00FF00; text-align: center"
|CC
|CC
-
|(rbs - june/foss) :: ter in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|(Ptet :: (rbs :: EYFP)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
| BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|
|
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #FFCC00; text-align: center"
+
|- style="background: #006600; text-align: center; color:white;"
|CC
|CC
-
|(rbs - fecA) :: ((rbs - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|((Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|
|
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #FFCC00; text-align: center"
+
|- style="background: #006600; text-align: center; color:white;"
-
|CC
+
|CC <span/ id="tolr2">
-
|(rbs - fecA) :: ((Ompa Linker :: g3p) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
|
|
|??
|??
|BBa_????
|BBa_????
 +
|}
-
|- style="background: #FFCC00; text-align: center"
+
 
 +
 
 +
*'''Plasmid C'''
 +
 
 +
 
 +
{|
 +
|- style="background: #0d3e99; text-align: center; color:white;"
 +
|width=3%| Designer
 +
|width=40%| Parts description
 +
|width=25%| Corresponding BioBricks
 +
|width=25%| Status (last update)
 +
|width=3%| Plasmid #
 +
|width=4%| Registry
 +
 
 +
|- style="background: #9966FF; text-align: center; color:black;"
|CC
|CC
-
|(rbs - ClyA-GFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|(rbs-FecI :: rbs-FecR) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|BBa_??? :: BBa_???
-
|
+
|primers ordered but not received yet (10/08/09)
|??
|??
|BBa_????
|BBa_????
-
|- style="background: #FFCC00; text-align: center"
+
 
 +
|- style="background: #9966FF; text-align: center; color:black;"
|CC
|CC
-
|(rbs - ClyA-GFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|PfecA in [http://partsregistry.org/Part:BBa_J61002 Bba_J61002]
-
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|BBa_???
-
|
+
|vector digested and purified, Pfec A amplified but not digested (purified?) (10/08/09)
|??
|??
|BBa_????
|BBa_????
|}
|}

Latest revision as of 21:51, 21 October 2009

iGEM > Paris > Freezer > Constructions in progress


Constructions


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol


  • Plasmid A


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
CC (Pbad :: rbs - lacI - LVA) in pSB1A3???Pbad is on PSB2K3 Bba_I0500 :: BBa_J24679 in pSB2K3 pSB1A3 transformed, digested, PBad transformed and not digested well- LacI transformed and digested (10/08/09) ?? BBa_????
CC (Pbad :: rbs - lacI - LVA) :: ter in pSB1A3 BBa_??? :: BBa_B0014 in pSB2K3 ter transformed, digested and purified (10/08/09) ?? BBa_????
CC ((Pbad :: rbs - lacI - LVA) :: RFP) in pSB1A3 BBa_??? :: BBa_K093005 in pSB2K3 pSB2K3 transformed and digested, RFP : primers designed but not yet ordered (10/08/09) ?? BBa_????


CC (((Pbad :: rbs - lacI - LVA) :: RFP) :: ter) in pSB1A3 BBa_??? :: BBa_B0014 in pSB2K3 ?? BBa_????


CC (OmpA Linker :: g3p) in pSB2K3 BBa_K103006 :: BBa_??? in pSB2K3 OmpA linker standardisé - OmpA amplified, not purified - Same for g3p (10/08/09) ?? BBa_????
(g3p :: clyA) in pSB2K3 BBa_??? :: BBa_??? in pSB2K3 autre possibilité (souf)- 1 primer missing for C term fusion with clyA (10/08/09) ?? BBa_????
CC! ((OmpA Linker :: g3p) :: ter) in pSB2K3 BBa_??? :: BBa_B0014 in pSB2K3 ?? BBa_????
CC! ((???autotransporter??? - jun/foss) :: ter) in pSB2K3 BBa_??? :: BBa_B0014 in pSB2K3 jun/foss has to be synthetized & autotransporter to be found!!!! (10/08/09) ?? BBa_????
CC! ((rbs - fecA) :: ((rbs - jun/foss) :: ter)) in pSB2K3 BBa_??? :: BBa_??? in pSB2K3 FecA : oligo ordered but not received yet (10/08/09) ?? BBa_????
CC! ((rbs - fecA) :: ((Ompa Linker :: g3p) :: ter)) in pSB2K3 BBa_??? :: BBa_??? in pSB2K3 ?? BBa_????
CC ((rbs - ClyA :: RFP) in pSB2K3 BBa_??? :: BBa_???? in pSB2K3 modif RFP (souf)- some troubles with amplification of clyA fusion C term (10/08/09) ?? BBa_????
CC ((rbs-RFP :: clyA) in pSB2K3 BBa_??? :: BBa_???? in pSB2K3 modif RFP (souf) - fusion N term de cly A (autre possibilité)- we still miss 1 primer for clyA N term fusion (10/08/09) ?? BBa_????
CC! ((rbs - ClyA :: RFP)) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in pSB2K3 BBa_??? :: BBa_???? in pSB2K3 modif RFP (souf) ?? BBa_????
CC! ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in pSB2K3 BBa_??? :: BBa_???? in pSB2K3 modif RFP (souf) ?? BBa_????
CC! (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) in pSB2K3 Bba_I0500 :: BBa_???? in pSB2K3 ?? BBa_????
CC! (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) in pSB2K3 BBa_??? :: BBa_???? in pSB2K3 ?? BBa_????
CC! ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in pSB2K3 BBa_??? :: BBa_???? in pSB2K3 2*Pbad et 2*ter?? (souf) ?? BBa_????
CC! ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in pSB2K3 BBa_??? :: BBa_???? in pSB2K3 ?? BBa_????



  • Plasmid B


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
GC ((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3 BBa_blabla :: BBa_tralala in truc Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09) 12 BBa_pouet pouet
CC (Plac :: rbs-tetR) in pSB1A3 Bba_I14032 :: BBa_K136050 in pSB1A3 both have been digested and purified, ligation in progress (10/08/09) ?? BBa_????
CC ((Plac :: rbs-tetR) :: ter) in pSB1A3 BBa_???? :: BBa_B0014 in pSB1A3 ?? BBa_K257015
CC (ter :: ((Plac :: rbs-tetR) :: ter)) in pSB1A3 BBa_B0014 :: BBa_K257015 in pSB1A3 ?? BBa_????
CL (rbs - OmpAsignal :: TolR II) BBa_K257006 ::BBa_K257005 in pSB1A3 OmpA signal and TolRII amplified, but neither digested nor purified (10/08/09) ?? BBa_????
CC (Ptet :: (rbs - OmpAsignal :: TolRII)) in pSB1A3 Bba_R0040 :: BBa_K257006 ::BBa_K257005 in pSB1A3 Ptet digested and purified (10/08/09) ?? BBa_????
CC (rbs :: EYFP) in pSB1A3 BBa_??? :: BBa_E0030 in pSB1A3 y a t-il vraiment 2 biobricks ou bien met-on le RBS direct dans des oligos pour RFP? (souf) biobrick to find or primers to design (10/08/09) ?? BBa_????
CC (Ptet :: (rbs :: EYFP)) in pSB1A3 Bba_R0040 :: BBa_??? in pSB1A3 ?? BBa_????
CC ((Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in pSB1A3 BBa_??? :: BBa_??? in pSB1A3 ?? BBa_????
CC ((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in pSB1A3 BBa_??? :: BBa_??? in pSB1A3 ?? BBa_????


  • Plasmid C


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
CC (rbs-FecI :: rbs-FecR) in pSB2K3 BBa_??? :: BBa_??? primers ordered but not received yet (10/08/09) ?? BBa_????


CC PfecA in Bba_J61002 BBa_??? vector digested and purified, Pfec A amplified but not digested (purified?) (10/08/09) ?? BBa_????

Retrieved from "http://2009.igem.org/Team:Paris/constructions"