Team:UNICAMP-Brazil/Coliguard/Results

From 2009.igem.org

(Difference between revisions)
(The Coliguard - Results)
(The Coliguard - Results)
Line 16: Line 16:
After the confirmation of the construction we characterized the part through the test that is described in the Notebook. [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_15#Testing_the_new_device Click here for details.]
After the confirmation of the construction we characterized the part through the test that is described in the Notebook. [https://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/October_15#Testing_the_new_device Click here for details.]
 +
We confirmed that our part really works as expected!!!!
 +
 +
The OD measurements showed a decrease on the growth of induced strains transformed with BBa_K284022, in comparsion with induced strains transformed with BBa_K112806 (without the promoter) and the non-induced ones. The lysis of the cells expressing T4 endolysin can be visualized in the picture below.
 +
 +
[[Image:cell-lysis.png|500px|center]]
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 02:44, 22 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif


The Coliguard - Results

Test and characterization of device BBa_K284022

We concluded the construction of the device BBa_K284022.

We confirmed and characterized the device. The results are shown next:

New biobrick.JPG

We confirm the construction through PCR and restriction analysis. Click here for details.

After the confirmation of the construction we characterized the part through the test that is described in the Notebook. Click here for details.

We confirmed that our part really works as expected!!!!

The OD measurements showed a decrease on the growth of induced strains transformed with BBa_K284022, in comparsion with induced strains transformed with BBa_K112806 (without the promoter) and the non-induced ones. The lysis of the cells expressing T4 endolysin can be visualized in the picture below.

Cell-lysis.png