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ColiGuard
Recovering the BioBricks
Today we ressuspended the chosen BioBricks according to the standard protocol. Each undergraduated student was responsible to ressuspend a set of BioBricks. All of them were transfomed into electrocompetent E. coli (DH10B) according to Protocol 3. The cells were then plated in LB media containing the appropriate antibiotic.
Marcelo
Part:BBa_B0030 - RBS.1 (strong) -- modified from R. Weiss
Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030
Luige
Part:BBa_J61100 - Ribosome Binding Site Family Member
Part:BBa_J61107 - Ribosome Binding Site Family Member
Marcos
Part:BBa_B0010 - T1 from E. coli rrnB
Part:BBa_B0014 - double terminator
Leonardo
Part:BBa_B0015 - double terminator (B0010-B0012)
Part:BBa_R0040 - TetR repressible promoter
Part:BBa_J23100 - constitutive promoter family member
Part:BBa_J23107 - constitutive promoter family member
Fabiana
Part:BBa_J23109 - constitutive promoter family member
Part:BBa_J31007 - tetracycline resistance protein TetA(C)
Part:BBa_J31003 - kanamycin resistance forward
Part:BBa_E0040 - green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)
Victor
Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from Discosoma striata
Part:pSB1A3 - High copy BioBrick assembly plasmid
Part:pSB1AK3 - High copy BioBrick assembly plasmid
Part:pSB3K3 - Low to medium copy BioBrick standard vector
Part:pSB6A1 - pBR322 converted to BioBrick vector (formally BBa_I739201)
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