Team:UNICAMP-Brazil/Notebooks/August 14

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{{:Team:UNICAMP-Brazil/inc calendar}}
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== YeastGuard: recovering BBa_J63002==
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__NOTOC__
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- Today we resuspended BBa_J63002 again, but this time from the plate.
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==''' ColiGuard '''==
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- We transformed the biobrick into ''E. coli'' DH10B competent cells and plated on Kan and Amp plates, to find out in which plate it will work.
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==== Results of transformation ====
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'''Taís'''
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*<p style=”text-align:justify;”>Today we noticed that just two transformations performed yesterday were succesfull:</p>
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- BBa_K131009
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==Coliguard: Results of transformation==
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- BBa_K091109
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Today we noticed that just two transformations performed yesterday were succesfull.
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''-BBa_K131009''
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*<p style=”text-align:justify;”>So, we performed plasmid extraction according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. We ran an agarose gel to comprove extraction.</p>
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-BBa_K091109
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[[Image:Agarose_Miniprep_col_E2_e_luxS.jpg‎ |center|‎]]
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''Luige''
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==''' YeastGuard '''==
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==== Recovering Biobricks: the relutant one====
 +
 
 +
* Today we resuspended BBa_J63002 again, but this time from the plate.
 +
 
 +
* We transformed the biobrick into ''E. coli'' DH10B competent cells and plated on Kan and Amp plates, to find out in which plate it will work.
 +
 
 +
''Taís''
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So, we performed plasmid extraction according to protocol 2 (see prototol section). We run a agarose gel to prove extraction.
 
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[[Image:Agarose_Miniprep_col_E2_e_luxS.jpg‎ |center|‎]]
 
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'''Luige.'''
 
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 00:48, 22 October 2009

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ColiGuard

Results of transformation

  • Today we noticed that just two transformations performed yesterday were succesfull:

- BBa_K131009

- BBa_K091109

  • So, we performed plasmid extraction according to Protocol 2. We ran an agarose gel to comprove extraction.

‎

Luige

YeastGuard

Recovering Biobricks: the relutant one

  • Today we resuspended BBa_J63002 again, but this time from the plate.
  • We transformed the biobrick into E. coli DH10B competent cells and plated on Kan and Amp plates, to find out in which plate it will work.

Taís






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