Team:UNICAMP-Brazil/Notebooks/August 14

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-BBa_K091109
-BBa_K091109
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So, we performed plasmid extraction according to Protocol 2 (see prototols section). We run a agarose gel to prove extraction.
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So, we performed plasmid extraction according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. We run a agarose gel to prove extraction.
[[Image:Agarose_Miniprep_col_E2_e_luxS.jpg‎ |center|‎]]
[[Image:Agarose_Miniprep_col_E2_e_luxS.jpg‎ |center|‎]]

Revision as of 23:01, 3 October 2009

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ColiGuard

Results of transformation

Today we noticed that just two transformations performed yesterday were succesfull.

-BBa_K131009

-BBa_K091109

So, we performed plasmid extraction according to Protocol 2. We run a agarose gel to prove extraction.

‎

Luige

YeastGuard

Recovering BBa_J63002

  • Today we resuspended BBa_J63002 again, but this time from the plate.
  • We transformed the biobrick into E. coli DH10B competent cells and plated on Kan and Amp plates, to find out in which plate it will work.

Taís






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