Team:UNICAMP-Brazil/Notebooks/August 14

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YeastGuard: recovering BBa_J63002

- Today we resuspended BBa_J63002 again, but this time from the plate.

- We transformed the biobrick into E. coli DH10B competent cells and plated on Kan and Amp plates, to find out in which plate it will work.

Taís


Coliguard: Results of transformation

Today we noticed that just two transformations performed yesterday were succesfull.

-BBa_K131009

-BBa_K091109

So, we performed plasmid extraction according to protocol 2 (see prototol section). We run a agarose gel to prove extraction.

Luige.