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Team:UNICAMP-Brazil/Notebooks/August 21
From 2009.igem.org
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Miniprep, digestion and ligation of RBS | Miniprep, digestion and ligation of RBS | ||
| - | * Today we perfomed miniprep from the RBS transformed ''E. coli''. The fragment size was correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11] without purifying. It worked. | + | * Today we perfomed miniprep from the RBS transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. The fragment size was correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11] without purifying. It worked. |
* After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol 11. | * After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol 11. | ||
Revision as of 02:19, 21 October 2009
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