Team:UNICAMP-Brazil/Notebooks/August 21

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YeastGuard

Recovered biobricks

Miniprep, digestion and ligation of RBS

  • Today we perfomed miniprep from the RBS transformed E. coli. The fragment size was correct and we decided to digest it ([1]) without purifying. It worked.
  • After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol 11.

Raíssa