Team:UNICAMP-Brazil/Notebooks/August 31

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(→Quantification)
(→Quantification)
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We have verified the concentration of RNA we got in the RNA extractions from the ''G. gallus'' organs. The following table shows the results.
We have verified the concentration of RNA we got in the RNA extractions from the ''G. gallus'' organs. The following table shows the results.
-
{| class="wikitable" border="0"
+
{| class="wikitable" border="1"
|-
|-
-
!Samples
+
!Samples !!ng/uL !!260/280 !!260/230  
-
!ng/uL
+
-
!260/280
+
-
!260/230
+
|-
|-
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!Blood 1
+
!Blood 1 !!743.42 !!2.01 !!1.78
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!743.42
+
-
!2.01
+
-
!1.78
+
|-
|-
-
!Blood 2
+
!Blood 2 !!604.84 !!1.99 !!1.54
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!604.84
+
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!1.99
+
-
!1.54
+
|-
|-
-
!Blood 3
+
!Blood 3 !!1814.10 !!1.97 !!1.88
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!1814.10
+
-
!1.97
+
-
!1.88
+
|-
|-
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!Spleen 1
+
!Spleen 1 !!3607.90 !!1.96 !!1.57
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!3607.90
+
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!1.96
+
-
!1.57
+
|-
|-
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!Spleen 2
+
!Spleen 2 !!3715.95 !!1.94 !!1.55
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!3715.95
+
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!1.94
+
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!1.55
+
|-
|-
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!Spleen 3
+
!Spleen 3 !!4213.93 !!1.78 !!1.69
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!4213.93
+
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!1.78
+
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!1.69
+
|-
|-
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!Spleen 4
+
!Spleen 4 !!4243.37 !!1.76 !!1.69
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!4243.37
+
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!1.76
+
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!1.69
+
|-
|-
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!Oviduct 1
+
!Oviduct 1 !!4362.04 !!1.66 !!1.41
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!4362.04
+
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!1.66
+
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!1.41
+
|-
|-
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!Oviduct 2
+
!Oviduct 2 !!3498.69 !!1.87 !!1.16
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!3498.69
+
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!1.87
+
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!1.16
+
|-
|-
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!Oviduct 3
+
!Oviduct 3 !!3601.43 !!1.84 !!1.13
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!3601.43
+
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!1.84
+
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!1.13
+
|-
|-
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!Oviduct 4
+
!Oviduct 4 !!3568.66 !!1.84 !!1.12
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!3568.66
+
-
!1.84
+
-
!1.12
+
|}
|}
The concentration values are ok, but the ratios 260/280 and 260/230 are not that good. Let’s find out what we see in the agarose gel.
The concentration values are ok, but the ratios 260/280 and 260/230 are not that good. Let’s find out what we see in the agarose gel.

Revision as of 19:54, 7 September 2009

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YeastGuard

Gallus gallus RNA

Quantification

We have verified the concentration of RNA we got in the RNA extractions from the G. gallus organs. The following table shows the results.

Samples ng/uL 260/280 260/230
Blood 1 743.42 2.01 1.78
Blood 2 604.84 1.99 1.54
Blood 3 1814.10 1.97 1.88
Spleen 1 3607.90 1.96 1.57
Spleen 2 3715.95 1.94 1.55
Spleen 3 4213.93 1.78 1.69
Spleen 4 4243.37 1.76 1.69
Oviduct 1 4362.04 1.66 1.41
Oviduct 2 3498.69 1.87 1.16
Oviduct 3 3601.43 1.84 1.13
Oviduct 4 3568.66 1.84 1.12

The concentration values are ok, but the ratios 260/280 and 260/230 are not that good. Let’s find out what we see in the agarose gel. Raíssa

Agarose gel

Taís

Terminator Biobrick

Today we perfomed miniprep of the Terminator Biobrick transformed E. coli, according to Protocol 2 (see protocols section).

Wesley




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