Team:UNICAMP-Brazil/Notebooks/July 25

From 2009.igem.org

(Difference between revisions)
(Replacing page with '{{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} {{:Team:UNICAMP-Brazil/inc_rodape}}')
(Primer design)
 
(5 intermediate revisions not shown)
Line 2: Line 2:
{{:Team:UNICAMP-Brazil/inc calendar}}
{{:Team:UNICAMP-Brazil/inc calendar}}
 +
__NOTOC__
 +
 +
==''' MicroGuards '''==
 +
 +
====Primer design====
 +
 +
These are the primers that we are going to use in our project. How long will it take until they´re sent to us?? :)
 +
 +
 +
 +
Col-R CTACTAGTACCAGAAATTCAGCCTCGGTA
 +
 +
Anticol-F TTCTAGAGGTGGTAGGGAACGCTTTGAA
 +
 +
Anticol-R CTACTAGTAACCGGCATTCATATTTCAGC
 +
 +
HLY-F TTCTAGAGATAAAGATGGTCGAGA
 +
 +
HLY-R CTACTAGTCTAACGTTCACGTAAACTT
 +
 +
LYS-F TTCTAGAATGGTTGAAATCAATAATC
 +
 +
LYS-R CTACTAGTTACATCAATCTCTCTGACC
 +
 +
FinOF-XbaI TTCTAGAGATGACAGAGCAGAAACGACCGG
 +
 +
FinOR-SpeI CTACTAGTATTATTTCTCATCAAGCACGGCC
 +
 +
FinPF-XbaI TTCTAGAGTGACGAGCATGTTTTTGTTGAA
 +
 +
FinPR-SpeI CTACTAGTAAAAATCGCCGATGCAGGGAGAC
 +
 +
Ppy-F-1 TTCTAGAGACGAGAAGGCTATGTGTATCA
 +
 +
Ppy-F-2 TTCTAGAGTTTCCGTCTATTTACCTTTTCTG
 +
 +
Ppy-R CTACTAGTATAACACCTCCCGCTGTTTAT
 +
 +
KlJEN1-F TTCTAGATCAACCATGAATAACAACAAT
 +
 +
KlJEN1-R CTACTAGTATAATGCTATGGTGCGTGT
 +
 +
Prom-KlJEN1-F TTCTAGATGATTGGCAAACCTGTTGT
 +
 +
Prom-KlJEN1-R CTACTAGTGGTTGAAATCTGTTTTGGTTTT
 +
 +
Prom-KlDLD-F TTCTAGAGGTCGCTCTTTCTCTCTCTCAT
 +
 +
Prom-KlDLD-R CTACTAGTTGAGTTGCGTTTCTGGTCTTT
 +
 +
GgLYSO_F TTCTAGAATGAGGTCTTTGCTAATCTTGG
 +
 +
GgLYSO_R CTACTAGTTTTTAATGAGGGATGCGTTT
 +
 +
Cre-F TCCAATTTACTGACCGTACACC
 +
 +
FSTK_UP_VF2 CACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTTGCCACCTGACGTCTAAGAA
 +
 +
FSTK_UP_VR TACAGGCTTTTCCTGCCCGATGCTAAAACGGACAACAGTACCATTACCGCCTTTGAGTGAGC
 +
 +
FSTK_DOWN_VF2 TCGTATGCCGGATAAGGCGCGGTAGCGTCGCATCCGGCACTCTGCCACCTGACGTCTAAGAA
 +
 +
FSTK_DOWN_VR AAATTACGTCACTCCCGATCGGGAGTTCCCTCTACTCTCTATATTACCGCCTTTGAGTGAGC
 +
 +
Slippage_Cre-R TGGTGTACGGTCAGTAAATTGGAGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTCAT

Latest revision as of 16:51, 7 September 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

MicroGuards

Primer design

These are the primers that we are going to use in our project. How long will it take until they´re sent to us?? :)


Col-R CTACTAGTACCAGAAATTCAGCCTCGGTA

Anticol-F TTCTAGAGGTGGTAGGGAACGCTTTGAA

Anticol-R CTACTAGTAACCGGCATTCATATTTCAGC

HLY-F TTCTAGAGATAAAGATGGTCGAGA

HLY-R CTACTAGTCTAACGTTCACGTAAACTT

LYS-F TTCTAGAATGGTTGAAATCAATAATC

LYS-R CTACTAGTTACATCAATCTCTCTGACC

FinOF-XbaI TTCTAGAGATGACAGAGCAGAAACGACCGG

FinOR-SpeI CTACTAGTATTATTTCTCATCAAGCACGGCC

FinPF-XbaI TTCTAGAGTGACGAGCATGTTTTTGTTGAA

FinPR-SpeI CTACTAGTAAAAATCGCCGATGCAGGGAGAC

Ppy-F-1 TTCTAGAGACGAGAAGGCTATGTGTATCA

Ppy-F-2 TTCTAGAGTTTCCGTCTATTTACCTTTTCTG

Ppy-R CTACTAGTATAACACCTCCCGCTGTTTAT

KlJEN1-F TTCTAGATCAACCATGAATAACAACAAT

KlJEN1-R CTACTAGTATAATGCTATGGTGCGTGT

Prom-KlJEN1-F TTCTAGATGATTGGCAAACCTGTTGT

Prom-KlJEN1-R CTACTAGTGGTTGAAATCTGTTTTGGTTTT

Prom-KlDLD-F TTCTAGAGGTCGCTCTTTCTCTCTCTCAT

Prom-KlDLD-R CTACTAGTTGAGTTGCGTTTCTGGTCTTT

GgLYSO_F TTCTAGAATGAGGTCTTTGCTAATCTTGG

GgLYSO_R CTACTAGTTTTTAATGAGGGATGCGTTT

Cre-F TCCAATTTACTGACCGTACACC

FSTK_UP_VF2 CACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTTGCCACCTGACGTCTAAGAA

FSTK_UP_VR TACAGGCTTTTCCTGCCCGATGCTAAAACGGACAACAGTACCATTACCGCCTTTGAGTGAGC

FSTK_DOWN_VF2 TCGTATGCCGGATAAGGCGCGGTAGCGTCGCATCCGGCACTCTGCCACCTGACGTCTAAGAA

FSTK_DOWN_VR AAATTACGTCACTCCCGATCGGGAGTTCCCTCTACTCTCTATATTACCGCCTTTGAGTGAGC

Slippage_Cre-R TGGTGTACGGTCAGTAAATTGGAGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTCAT