Team:UNICAMP-Brazil/Notebooks/October 15

From 2009.igem.org

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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====New strategy: pGEM==== *<p style=”text-align:justify;”>We transformed...)
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====New strategy: pGEM====
====New strategy: pGEM====
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*<p style=”text-align:justify;”>We sent 4 biobricks to iGEM today: pJEN1, pDLD, Lysozyme, and the devices Adh1+YFP and Adh1+Lysozyme. =)</p>
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====Yeast experiments====
*<p style=”text-align:justify;”>We transformed the YEP+Adh1-lysozyme ligation reaction in competent ''E. coli'' and plated in LB+Amp media. Hope to have colonies later!</p>
*<p style=”text-align:justify;”>We transformed the YEP+Adh1-lysozyme ligation reaction in competent ''E. coli'' and plated in LB+Amp media. Hope to have colonies later!</p>
*<p style=”text-align:justify;”>We did miniprep of YEP+Adh1-YFP and the following devices: b) pJEN1+YFP, c) pJEN1+Lys, d) pDLD+YFP, e) pDLD+Lys.</p>
*<p style=”text-align:justify;”>We did miniprep of YEP+Adh1-YFP and the following devices: b) pJEN1+YFP, c) pJEN1+Lys, d) pDLD+YFP, e) pDLD+Lys.</p>
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*<p style=”text-align:justify;”>We sent 4 biobricks to iGEM today: pJEN1, pDLD, Lysozyme, and the devices Adh1+YFP and Adh1+Lysozyme. =)</p>
 

Revision as of 03:31, 21 October 2009

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YeastGuard

New strategy: pGEM

  • We sent 4 biobricks to iGEM today: pJEN1, pDLD, Lysozyme, and the devices Adh1+YFP and Adh1+Lysozyme. =)


Yeast experiments

  • We transformed the YEP+Adh1-lysozyme ligation reaction in competent E. coli and plated in LB+Amp media. Hope to have colonies later!

  • We did miniprep of YEP+Adh1-YFP and the following devices: b) pJEN1+YFP, c) pJEN1+Lys, d) pDLD+YFP, e) pDLD+Lys.


Raíssa and Taís