Team:UNICAMP-Brazil/Notebooks/September 1

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''Taís''
''Taís''
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====RBS+CFP ligation====
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====RBS+CFP Digestion====
We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Restriction_reaction Protocol 14]. The digestion lasted four hours and we used less plasmid comparing to the first digestion.
We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Restriction_reaction Protocol 14]. The digestion lasted four hours and we used less plasmid comparing to the first digestion.

Revision as of 18:22, 21 October 2009

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YeastGuard

Gallus gallus cDNA synthesis

We decided to make cDNA synthesis of the 4 samples of RNA (Blood1, Blood3, Spleen1 and Oviduct2). We used both pDN6 and oligodT primers for the synthesis.

Taís

RBS+CFP Digestion

We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed Protocol 14. The digestion lasted four hours and we used less plasmid comparing to the first digestion.

Wesley




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