Team:UNICAMP-Brazil/Notebooks/September 24

ColiGuard

E. coli transformation with F plasmid

• E. coli DH10β electrocompetent cells were transformed using the recircularized F plasmid, according to protocol 4.
• After the incubation time, 100 μl of medium containing the transformed cells were plated in LB + ampicilin + Xgal culture plates, and incubated O/N at 37°C.

Gabriel

finO and finP - Still Trying to Confirm our Biobricks

• After the miniprep procedure, we performed PCRs with finO and finP's designed primer, in order to confirm that our inserted fragment is indeed contained in the vector.
• As we had already got an amplified fragment of expected size from those colonies, we expect that those PCRs results in a successful amplification of our fragments. Moreover, as we aimed in reducing the amount of genomic DNA, we should got less inespecific amplifications.

Marcelo