Team:UNICAMP-Brazil/Notebooks/September 24

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ColiGuard

E. coli transformation with F plasmid

  • E. coli DH10β electrocompetent cells were transformed using the recircularized F plasmid, according to Protocol 3.

  • After the incubation time, 100 μl of medium containing the transformed cells were plated in LB-AMP-Xgal culture plates, and incubated O/N at 37°C.

Gabriel


finO and finP - Still Trying to Confirm our Biobricks

  • After the miniprep procedure, we performed PCRs with finO and finP's designed primer, in order to confirm that our inserted fragment is indeed contained in the vector.

  • As we had already got an amplified fragment of expected size from those colonies, we expect that those PCRs results in a successful amplification of our fragments. Moreover, as we aimed in reducing the amount of genomic DNA, we should got less inespecific amplifications.


Marcelo

PY Promoter - Transformation

  • Today we transformed the ligations PY1 + pGEM and PY2 + pGEM into electrocompetent E. coli bacteria, strain DH10B. We followed Protocol 3 (Electroporation) without modifications.

  • After the transformation we plated the transformed cells in LB-AMP-Xgal plates, and let them grow at 37ºC for an O/N period.

Fabi and Léo


CeaB and CeiB: Miniprep and transformation

  • Today, we made Miniprep of the transformated cells and we made PCR again, so that avoids unspecific amplification. We didn’t get the band size expected again.


Luige