Team:UNICAMP-Brazil/Notebooks/September 6

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YeastGuard

New biobricks

Today we digested all our 4 new biobrick parts with both enzymes: Xba1 and Spe1 (Promoter of JEN1 from K. lactis; Promoter of DLD1 from K. lactis; Lysozyme from G. gallus; Gene JEN1 from K. lactis). We also digested the biofusion vector with the same enzymes for future ligation.

GEL

  • Digestions that worked:

- Promoter of JEN1 from K. lactis

- Promoter of DLD1 from K. lactis

- Lysozyme from G. gallus

  • Digestions that didnt work:

- Gene JEN1 from K. lactis

- Biofusion vector





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