Team:UNICAMP-Brazil/Protocols

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Protocols

1. Genomic DNA Extraction

2. Mini-Prep

3. Transformation

4. Preparation of electrocompetent E. coli

5. Yeast DNA Extraction

6. Yeast RNA Extraction

7. Purification of DNA fragments from agarose gels

8. Isolation of RNA using Trizol Reagent (Invitrogen)

9. SAP Dephosphorylation

10. CIAP Dephosphorylation

11. T4 DNA Ligase

12. Electroelution

pGEM cloning strategy

13. Preparation of electrocompetent S. cerevisiae

14. Digestion Reaction