Team:Wisconsin-Madison/Notebook Protocols

(Difference between revisions)

Revision as of 13:03, 19 October 2009

PROTOCOLS Plasmid Preparation by Alkaline Lysis Plasmid Preparation by Boiling Lysis Restriction Enzyme Mapping Transformation of Plasmids into Competent Cells Preparation of Competent Cells Overview of Cloning Protocol GREAT for New Teams! Polymerase Chain Reaction (PCR) Ligation Digestion - Cloning Inoculation of Plasmids in Liquid Media Loading Gel Protocol Plasmid Clean-up Sequencing Reaction Colony Polymerase Chain Reaction (PCR) Genomic DNA Extraction Safety

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 * [[Team:Wisconsin-Madison/Digestion - Cloning|Digestion - Cloning]]
 * [[Team:Wisconsin-Madison/Inoculation of Plasmids in Liquid Media|Inoculation of Plasmids in Liquid Media]]
-* [[Team:Wisconsin-Madison/Inoculation of Cyanobacteria|Inoculation of Cyanobacteria]]
 * [[Team:Wisconsin-Madison/Loading Gel Protocol|Loading Gel Protocol]]
 * [[Team:Wisconsin-Madison/Plasmid Clean-up|Plasmid Clean-up]]
-* [[Team:Wisconsin-Madison/Gel Extraction from Agar|Gel Extraction from Agar]]
 * [[Team:Wisconsin-Madison/Sequencing Reaction|Sequencing Reaction]]
 * [[Team:Wisconsin-Madison/Colony Polymerase Chain Reaction (PCR)|Colony Polymerase Chain Reaction (PCR)]]
 * [[Team:Wisconsin-Madison/Genomic DNA Extraction|Genomic DNA Extraction]]
 * [[Team:Wisconsin-Madison/Safety|Safety]]