Team:Wisconsin-Madison/Notebook Protocols

(Difference between revisions)

Latest revision as of 18:26, 20 October 2009

PROTOCOLS Plasmid Preparation by Alkaline Lysis Plasmid Preparation by Boiling Lysis Restriction Enzyme Mapping Transformation of Plasmids into Competent Cells Preparation of Competent Cells Overview of Cloning Protocol GREAT for New Teams! Polymerase Chain Reaction (PCR) Ligation Digestion - Cloning Loading Gel Protocol Sequencing Reaction Colony Polymerase Chain Reaction (PCR) Genomic DNA Extraction Safety

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 * [[Team:Wisconsin-Madison/Ligation|Ligation]]
 * [[Team:Wisconsin-Madison/Digestion - Cloning|Digestion - Cloning]]
-* [[Team:Wisconsin-Madison/Inoculation of Plasmids in Liquid Media|Inoculation of Plasmids in Liquid Media]]
 * [[Team:Wisconsin-Madison/Loading Gel Protocol|Loading Gel Protocol]]
-* [[Team:Wisconsin-Madison/Plasmid Clean-up|Plasmid Clean-up]]
 * [[Team:Wisconsin-Madison/Sequencing Reaction|Sequencing Reaction]]
 * [[Team:Wisconsin-Madison/Colony Polymerase Chain Reaction (PCR)|Colony Polymerase Chain Reaction (PCR)]]
 * [[Team:Wisconsin-Madison/Genomic DNA Extraction|Genomic DNA Extraction]]
 * [[Team:Wisconsin-Madison/Safety|Safety]]