UQ-Australia/15 September 2009

From 2009.igem.org

Notebook

July
M	T	W	T	F	S	S
		1	2	3	4	5
6	7	8	9	10	11	12
13	14	15	16	17	18	19
20	21	22	23	24	25	26
27	28	29	30	31

August
M	T	W	T	F	S	S
					1	2
3	4	5	6	7	8	9
10	11	12	13	14	15	16
17	18	19	20	21	22	23
24	25	26	27	28	29	30
31

September
M	T	W	T	F	S	S
	1	2	3	4	5	6
7	8	9	10	11	12	13
14	15	16	17	18	19	20
21	22	23	24	25	26	27
28	29	30

October
M	T	W	T	F	S	S
			1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	31

Mercury Vacuum Project

15/09/09 - Repeat Transformation of BBaI716101
The overnight transformation from 14/09/09 was unsuccessful as no growth was observed on plates. It is probable that the cell death plasmid component (Part BBa_P1010) is functioning normally as it was believed that this component was 'faulty'. The same protocol as on 14/09/09 was used for the transformation with an extra positive control for comparison. A well characterised plasmid PUC18 (ampicillin resistance) was used as the positive control.

Bioprecipitation Project

15/09/09 - Transformation with PXCK-K and PXCK-EL
PXCK-K and PXCK-EL plasmids were transformed into electrocompetent E. coli and left overnight at 37C in ampicillin plates (PXCK-K) or chloramphenicol plates (PXCK-EL).