Team:KULeuven/Freezer
From 2009.igem.org
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|dNTP mix||||ATP, GTP, TTP, CTP||||94||10μM||aug 10 | |dNTP mix||||ATP, GTP, TTP, CTP||||94||10μM||aug 10 | ||
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- | |{{kulpart| | + | |{{kulpart|BBa_J23110}}||{{kulpart|pSB1A2}}||medium constitutieve promoter||||||||july 23 |
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|ampicilline|||||||||||| | |ampicilline|||||||||||| | ||
|} | |} |
Revision as of 13:03, 1 September 2009
Contents |
Blue light receptor
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
MC4100 | / | strain of E.coli | july 24 | |||
2260 | / | forward primer for | 42,5µl | 20μM | aug 10 | |
2261 | / | reverse primer for | 45µl | 20μM | aug 10 | |
GFP + rbs + terminator | Miniprepped | 100μl | 85,6ng/μl | july 29 | ||
GFP + rbs + terminator | restricted with EcoRI and XbaI | 20μl | july 29 | |||
GFP + LVA-tag + terminator | restricted with EcoRI and XbaI | 30μl | 29,3 ng/μl | aug 14 | ||
kanamycine plasmide backbone | taken from plates | 13μl | aug 11 | |||
/ | Exact blue light promotor | pcr product | 20μl | 123,3 ng/μl | aug 13 | |
/ | constitutive promotor | taken from plates | 13μl | aug 11 | ||
/ | Exact blue light promotor | cut with EcoRI and SpeI | 26μl | 27 ng/μl | aug 12 | |
+ | LigA: Exact blue light promotor ligated with in | T4 DNA ligation | 18μl | aug 13 | ||
+ | Lig B: Exact blue light promotor ligated with in | T4 DNA ligation | 18μl | aug 14 | ||
+ | / | Lig A: Exact blue light promotor ligated with in (1,3,7,9) | cut with EcoRI and PstI | 4 x 18μl | aug 24 | |
+ | LigA: Exact blue light promotor ligated with in | Miniprep (4x) | 20μl | aug 18 | ||
constitutive promotor | miniprep | 10μl | 141,2ng/μl | aug 14 | ||
(A) | constitutive promotor | restriction digest with EcoRI en SpeI | 20μl | 20,8ng/μl | aug 24 | |
(B) | constitutive promotor | restriction digest with EcoRI en SpeI | 10μl | 12,8ng/μl | aug 24 | |
+ | LigA (1): Exact blue light promotor ligated with in | Miniprep | 45μl | 110,6ng/μl | aug 31 | |
+ | LigA (2): Exact blue light promotor ligated with in | Miniprep | 43μl | 76,7ng/μl | aug 31 | |
+ | LigA (3): Exact blue light promotor ligated with in | Miniprep | 44μl | 84,8ng/μl | aug 31 | |
+ | LigA (4): Exact blue light promotor ligated with in | Miniprep | 34μl | 31,7ng/μl | aug 31 | |
pSB1A2 | RBS + GFP + terminator | Miniprep | 45μl | 104,3ng/μl | aug 31 |
Key/antikey/lock
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
/ | GFP with LVA tag | purified | july 28 | |||
/ | tetR promoter | purified | july 28 |
vanillin synthesis
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
ech + rbs | purified plasmid (oranje) | 108,8 ng/µl | August 4 | |||
Sam5 + rbs, PstI site removed | purified plasmid (oranje) | 101,3 ng/µl | August 5 | |||
COMT | purified plasmid (oranje) | 122,9 ng/µl | August 5 | |||
fcs + rbs | purified plasmid (fcs pl.) | 29 µl | 57,2 ng/µl | August 12 | ||
Sam5 + rbs | purified plasmid (sam5 pl.) | 31 µl | 76,0 ng/µl | August 12 | ||
ech + rbs | purified plasmid (ech B) | 42,8 µl | 96,7 ng/µl | August 19 | ||
ech + rbs | purified plasmid (ech C) | 48 µl | 105,6 ng/µl | August 19 | ||
fcs + rbs | purified plasmid (fcs B) | 43,6 µl | 113,4 ng/µl | August 19 | ||
fcs + rbs | purified plasmid (fcs C) | 48 µl | 90,3 ng/µl | August 19 | ||
Sam5 + rbs | purified plasmid (sam5 B) | 44 µl | 125,0 ng/µl | August 19 | ||
Sam5 + rbs | purified plasmid (sam5 C) | 48 µl | 104,2 ng/µl | August 19 | ||
Sam8 + rbs | purified plasmid (sam8 B) | 40,1 µl | 63,2 ng/µl | August 19 | ||
sam8 + sam5 | ligation mix (SAMS) | August 24 | ||||
fcs + ech | ligation mix (EF) | August 24 | ||||
fcs + ech | purified ligation plasmid (EF I) | 48 µl | 53,9 ng/µl | August 4 | ||
fcs + ech | purified ligation plasmid (EF II) | 42 µl | 39,4 ng/µl | August 4 | ||
fcs + ech | purified ligation plasmid (EF III) | 39,9 µl | 57,1 ng/µl | August 4 | ||
sam8 + sam5 | purified ligation plasmid (SAMS I) | 48 µl | 76,8 ng/µl | August 4 | ||
sam8 + sam5 | purified ligation plasmid (SAMS II) | 35,3 | 83,4 ng/µl | August 4 | ||
sam8 + sam5 | purified ligation plasmid (SAMS III) | 39,3 µl | 61,5 ng/µl | August 4 | ||
sam8 + sam5 | purified ligation plasmid (SAMS II), not purified from gel | 5 µl | August 31 | |||
sam8 + sam5 | purified ligation plasmid (SAMS I), purified from gel | 25 µl | August 31 | |||
fcs + ech | purified ligation plasmid (EF II), purified from gel | 5 µl | August 31 |
vanillin receptor
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
2235 | / | forward primer for | 20μM | August 3 | ||
2238 | / | reverse primer for | 20μM | August 3 | ||
2235 | / | forward primer for | 100μM | August 3 | ||
2238 | / | reverse primer for | 100μM | August 3 | ||
2242 | / | forward primer for | 20μM | August 3 | ||
2243 | / | reverse primer for | 20μM | August 3 | ||
2242 | / | forward primer for | 100μM | August 3 | ||
2243 | / | reverse primer for | 100μM | August 3 | ||
2244 | / | forward primer for | 20μM | August 3 | ||
2245 | / | reverse primer for | 20μM | August 3 | ||
2244 | / | forward primer for | 100μM | August 3 | ||
2245 | / | reverse primer for | 100μM | August 3 | ||
2246 | / | forward primer for | 20μM | August 3 | ||
2247 | / | reverse primer for | 20μM | August 3 | ||
2246 | / | forward primer for | 100μM | August 3 | ||
2247 | / | reverse primer for | 100μM | August 3 | ||
2288 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
2289 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
2288 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
2289 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
2290 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
2291 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
2290 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
2291 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
2292 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
2293 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
2292 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
2293 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
2294 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
2295 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
2294 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
2295 | / | reverse primer for mutagenesis for | 100μM | August 8 |
miscellaneous
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
dNTP mix | ATP, GTP, TTP, CTP | 94 | 10μM | aug 10 | ||
medium constitutieve promoter | july 23 | |||||
ampicilline |
Categories: Team:KULeuven/BioBricks | Team:KULeuven/BioBricks/BBa K238013 | Team:KULeuven/BioBricks/BBa E0240 | Team:KULeuven/BioBricks/pSB1A2 | Team:KULeuven/BioBricks/BBa K145015 | Team:KULeuven/BioBricks/pSB3K3 | Team:KULeuven/BioBricks/BBa J23101 | Team:KULeuven/BioBricks/pSB1A3 | Team:KULeuven/BioBricks/BBa K145201 | Team:KULeuven/BioBricks/BBa I742113 | Team:KULeuven/BioBricks/BBa K238007 | Team:KULeuven/BioBricks/BBa I742107 | Team:KULeuven/BioBricks/BBa I742115 | Team:KULeuven/BioBricks/BBa I742106 | Team:KULeuven/BioBricks/BBa K238008 | Team:KULeuven/BioBricks/BBa K238009 | Team:KULeuven/BioBricks/BBa K238010 | Team:KULeuven/BioBricks/BBa K238011 | Team:KULeuven/BioBricks/BBa J23110