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Team:Cambridge/Project/CA03
From 2009.igem.org
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== TESTING AND CHARACTERISATION == | == TESTING AND CHARACTERISATION == | ||
=== In vivo expression of our devices in E.coli MG1655 === | === In vivo expression of our devices in E.coli MG1655 === | ||
| - | Our preliminary research indicated that carotenoids production in E.coli TOP10 was very weak. We decided to use another strain, E.coli MG1655, for in vivo expression of our devices. The amount of carotenoids produced became visibly higher. Generally, the colour became visible after incubation for about 12 hours. | + | Our preliminary research indicated that carotenoids production in ''E.coli'' TOP10 was very weak. We decided to use another strain, ''E.coli'' MG1655, for in vivo expression of our devices. The amount of carotenoids produced became visibly higher. Generally, the colour became visible after incubation for about 12 hours. |
''Lycopene-producing device (constitutive)'' | ''Lycopene-producing device (constitutive)'' | ||
| - | [[Image:Cam09_lyco_1.jpg| | + | [[Image:Cam09_lyco_1.jpg|''E.coli'' MG1655 transformed with K274110 and grown on agar plate overnight.]] |
<!--Do not remove the first and last lines in this page!-->{{Template:CambridgeBottom}} | <!--Do not remove the first and last lines in this page!-->{{Template:CambridgeBottom}} | ||
Revision as of 02:44, 18 October 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Carotenoids
TESTING AND CHARACTERISATION
In vivo expression of our devices in E.coli MG1655
Our preliminary research indicated that carotenoids production in E.coli TOP10 was very weak. We decided to use another strain, E.coli MG1655, for in vivo expression of our devices. The amount of carotenoids produced became visibly higher. Generally, the colour became visible after incubation for about 12 hours.
Lycopene-producing device (constitutive)
