Team:KULeuven/14 July 2009

From 2009.igem.org

Revision as of 11:56, 14 July 2009 by JochemDeen (Talk | contribs)

Contents

Progress

To do:

  • Strain selection
    • Odor-free E.coli available on partsregistry () [ordered]
  • Order iobricks for Vanillin detection [Done]
  • Primer selection for the promotor [Done]
    • Send mail to Institut for Biologie-Mikrobiologie ([http://www.ncbi.nlm.nih.gov/pubmed/19240136 The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli]) [Done]
  • Biobricks for vanillin synthesis
    • Send mail to University of Tuscia [Done]
    • Order:
      • (sam8 (tyrosine-ammonia lyase) coding sequence) [on hold]
      • (sam5 (coumarate hydroxylase) coding sequence) [on hold]
      • (COMT gene with ribosome binding site) [Available in kit]

Extra info on Vanillin synthesis

http://www.ncbi.nlm.nih.gov/pubmed/16232583 1 Construction of recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for ferulic acid biotransformation to vanillin, B C Okeke and V Venturi ,J Biosci Bioeng. 1999; 88(1): 103–106.

Concept

Missbluevanilla.jpg

Drylab

Wetlab

Experiment Name

Description

Elaboration

Results and conclusions

Remarks