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Team:KULeuven/22 July 2009
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There are two solutions to this problem: | There are two solutions to this problem: | ||
# Construct a different vanillin receptor | # Construct a different vanillin receptor | ||
| - | #* Using VirA, a phenol receptor in ''agrobacterium'' . | + | #* Using VirA/VirG, a phenol receptor in ''agrobacterium'' . |
# Detect an alternative substance that has a linear relationship with the vanillin concentration | # Detect an alternative substance that has a linear relationship with the vanillin concentration | ||
#* Vanillin inhibits the Ahl receptor | #* Vanillin inhibits the Ahl receptor | ||
#* Synthesis of another signaling molecule that will be sensed | #* Synthesis of another signaling molecule that will be sensed | ||
Revision as of 14:40, 22 July 2009
Project progress
Wet Lab
Project
The Vanillin Receptor is not usable, Madrid also tried to use and ran into all sorts of problems: Problems span from the binding of the periplasmic protein to the vanillin molecule, to the function of the fusion protein, to the activation of the ompR. There are two solutions to this problem:
- Construct a different vanillin receptor
- Using VirA/VirG, a phenol receptor in agrobacterium .
- Detect an alternative substance that has a linear relationship with the vanillin concentration
- Vanillin inhibits the Ahl receptor
- Synthesis of another signaling molecule that will be sensed
