Team:KULeuven/Freezer

From 2009.igem.org

(Difference between revisions)
(→vanillin synthesis)
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|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 B)||40,1 µl||63,2 ng/µl||August 19
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 B)||40,1 µl||63,2 ng/µl||August 19
|-
|-
-
|||pSB1A2||sam8 + sam5||ligation (SAMS 21)||||||August 24
+
|||pSB1A2||sam8 + sam5||ligation mix (SAMS 21)||||||August 24
|-
|-
-
|||pSB1A2||fcs + ech||ligation (EF 21)||||||August 24
+
|||pSB1A2||fcs + ech||ligation mix (EF 21)||||||August 24
 +
|-
 +
|||pSB1A2||fcs + ech||purified ligation plasmid (EF I)||48 µl||53,9 ng/µl||August 4
 +
|-
 +
|||pSB1A2||fcs + ech||purified ligation plasmid (EF II)||42 µl||39,4 ng/µl||August 4
 +
|-
 +
|||pSB1A2||fcs + ech||purified ligation plasmid (EF III)||39,9 µl||57,1 ng/µl||August 4
 +
|-
 +
|||pSB1A2||sam8 + sam5||purified ligation plasmid (SAMS I)||48 µl||76,8 ng/µl||August 4
 +
|-
 +
|||pSB1A2||sam8 + sam5||purified ligation plasmid (SAMS II)||35,3||83,4 ng/µl||August 4
 +
|-
 +
|||pSB1A2||sam8 + sam5||purified ligation plasmid (SAMS III)||39,3 µl||61,5 ng/µl||August 4
|-
|-
|}
|}

Revision as of 09:28, 26 August 2009

Contents

Blue light receptor

part plasmid description status μl left concentration date
MC4100 / strain of E.coli july 24
2171 / forward primer for 90µl 20μM july 24
2172 / reverse primer for 90µl 20μM july 24
2171 / forward primer for 100μM july 24
2172 / reverse primer for 100μM july 24
2260 / forward primer for 45µl 20μM aug 10
2261 / reverse primer for 45µl 20μM aug 10
2260 / forward primer for 100μM aug 10
2261 / reverse primer for 100μM aug 10
pSB1A2GFP + rbs + terminatorrestricted with EcoRI and XbaIjuly 29
pSB1A2GFP + LVA-tag + terminatorrestricted with EcoRI and XbaIjuly 29
/BLR promoter region (1:200 adn 1:500)restricted with EcoRIjuly 29
/PCR BLR promoter region (360bp)4 microcentrifuge tubesjuly 30
pSB3K3 kanamycine plasmide backbone taken from plates 13μl aug 11
/Exact blue light promotor pcr product / 123,3 ng/μl aug 13
/constitutive promotortaken from plates 13μl aug 11
/Exact blue light promotor cut with EcoRI and SpeI 26μl 27 ng/μl aug 12
/LigA: Exact blue light promotor ligated with E0240 in pSB1A2 T4 DNA ligation 18μl aug 13
/Lig B: Exact blue light promotor ligated with K145015 in pSB1A2 T4 DNA ligation 18μl aug 14
/Lig A: Exact blue light promotor ligated with E02040 in pSB1A2 (1,3,5,7,9) cut with EcoRI and PstI 5 x 18μl aug 18

Key/antikey/lock

part plasmid description status μl left concentration date
/GFP with LVA tagpurifiedjuly 28
/tetR promoter purifiedjuly 28

vanillin synthesis

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid (oranje)108,8 ng/µlAugust 4
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid (oranje)101,3 ng/µlAugust 5
pSB1A2COMTpurified plasmid (oranje)122,9 ng/µlAugust 5
pSB1A2fcs + rbspurified plasmid (fcs pl.)29 µl57,2 ng/µlAugust 12
pSB1A2Sam5 + rbspurified plasmid (sam5 pl.)31 µl76,0 ng/µlAugust 12
pSB1A2ech + rbspurified plasmid (ech B)42,8 µl96,7 ng/µlAugust 19
pSB1A2ech + rbspurified plasmid (ech C)48 µl105,6 ng/µlAugust 19
pSB1A2fcs + rbspurified plasmid (fcs B)43,6 µl113,4 ng/µlAugust 19
pSB1A2fcs + rbspurified plasmid (fcs C)48 µl90,3 ng/µlAugust 19
pSB1A2Sam5 + rbspurified plasmid (sam5 B)44 µl125,0 ng/µlAugust 19
pSB1A2Sam5 + rbspurified plasmid (sam5 C)48 µl104,2 ng/µlAugust 19
pSB1A2Sam8 + rbspurified plasmid (sam8 B)40,1 µl63,2 ng/µlAugust 19
pSB1A2sam8 + sam5ligation mix (SAMS 21)August 24
pSB1A2fcs + echligation mix (EF 21)August 24
pSB1A2fcs + echpurified ligation plasmid (EF I)48 µl53,9 ng/µlAugust 4
pSB1A2fcs + echpurified ligation plasmid (EF II)42 µl39,4 ng/µlAugust 4
pSB1A2fcs + echpurified ligation plasmid (EF III)39,9 µl57,1 ng/µlAugust 4
pSB1A2sam8 + sam5purified ligation plasmid (SAMS I)48 µl76,8 ng/µlAugust 4
pSB1A2sam8 + sam5purified ligation plasmid (SAMS II)35,383,4 ng/µlAugust 4
pSB1A2sam8 + sam5purified ligation plasmid (SAMS III)39,3 µl61,5 ng/µlAugust 4

vanillin receptor

part plasmid description status μl left concentration date
2235 / forward primer for 20μM August 3
2238 / reverse primer for 20μM August 3
2235 / forward primer for 100μM August 3
2238 / reverse primer for 100μM August 3
2242 / forward primer for 20μM August 3
2243 / reverse primer for 20μM August 3
2242 / forward primer for 100μM August 3
2243 / reverse primer for 100μM August 3
2244 / forward primer for 20μM August 3
2245 / reverse primer for 20μM August 3
2244 / forward primer for 100μM August 3
2245 / reverse primer for 100μM August 3
2246 / forward primer for 20μM August 3
2247 / reverse primer for 20μM August 3
2246 / forward primer for 100μM August 3
2247 / reverse primer for 100μM August 3
2288 / forward primer for mutagenesis for 20μM August 8
2289 / reverse primer for mutagenesis for 20μM August 8
2288 / forward primer for mutagenesis for 100μM August 8
2289 / reverse primer for mutagenesis for 100μM August 8
2290 / forward primer for mutagenesis for 20μM August 8
2291 / reverse primer for mutagenesis for 20μM August 8
2290 / forward primer for mutagenesis for 100μM August 8
2291 / reverse primer for mutagenesis for 100μM August 8
2292 / forward primer for mutagenesis for 20μM August 8
2293 / reverse primer for mutagenesis for 20μM August 8
2292 / forward primer for mutagenesis for 100μM August 8
2293 / reverse primer for mutagenesis for 100μM August 8
2294 / forward primer for mutagenesis for 20μM August 8
2295 / reverse primer for mutagenesis for 20μM August 8
2294 / forward primer for mutagenesis for 100μM August 8
2295 / reverse primer for mutagenesis for 100μM August 8

miscellaneous

part plasmid description status μl left concentration date
dNTP mixATP, GTP, TTP, CTP9410μMaug 10
pSB1A2medium constitutieve promoterjuly 23
ampicilline
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