Revision as of 11:52, 12 August 2009

Blue light receptor

part	plasmid	description	status	μl left	concentration	date
MC4100	/	strain of E.coli				july 24
2171	/	forward primer for		90µl	20μM	july 24
2172	/	reverse primer for		90µl	20μM	july 24
2171	/	forward primer for			100μM	july 24
2172	/	reverse primer for			100μM	july 24
2260	/	forward primer for		45µl	20μM	aug 10
2261	/	reverse primer for		45µl	20μM	aug 10
2260	/	forward primer for			100μM	aug 10
2261	/	reverse primer for			100μM	aug 10
	pSB1A2	GFP + rbs + terminator	restricted with EcoRI and XbaI			july 29
	/	BLR promoter region (1:200 adn 1:500)	restricted with EcoRI			july 29
	/	PCR BLR promoter region (360bp)	4 microcentrifuge tubes			july 30
Template:IGempart	/	kanamycine pasmide backbone	taken from plates	13μl		aug 11
Template:IGempart	/	constitutive promotor	taken from plates	13μl		aug 11
Template:IGempart	/	Exact blue light promotor	cut with EcoRI and SpeI	26μl		aug 12

part	plasmid	description	status	μl left	concentration	date
	/	GFP with LVA tag	purified			july 28
	/	tetR promoter	purified			july 28

plasmid	description	status	concentration	date
pSB1A2	ech + rbs	purified plasmid	108,8 ng/µl	August 4
pSB1A2	fcs + rbs	purified plasmid	113,9 ng/µl	August 4
pSB1A2	Sam5 + rbs	received from Edinburgh		August 3
pSB1A2	Sam5 + rbs, PstI site removed	purified plasmid	101,3 ng/µl	August 5
pSB1A2	Sam8 + rbs	purified plasmid	95,7 ng/µl	August 5
pSB1A2	Sam8	purified plasmid	81,5 ng/µl	August 5
pSB1A2	COMT	purified plasmid	122,9 ng/µl	August 5
pSB1A2	COMT + rbs	plasmid from registry		August 6
pSB1A2	ech + rbs	purified digest	16,9 ng/µl	August 6
	fcs + rbs	purified digest	16,6 ng/µl	August 6
pSB1A2	sam5 + rbs	purified digest	3,1 ng/µl	August 7
	sam8 + rbs	purified digest	3,4 ng/µl	August 7

plasmid	description	status	concentration	date
pSB1A2	ech + rbs	purified plasmid	108,8 ng/µl	August 4
pSB1A2	fcs + rbs	purified plasmid	113,9 ng/µl	August 4
pSB1A2	Sam5 + rbs	received from Edinburgh		August 3
pSB1A2	Sam5 + rbs, PstI site removed	purified plasmid	101,3 ng/µl	August 5

part	plasmid	description	μl left	concentration	date
dNTP mix		ATP, GTP, TTP, CTP	94	10μM	aug 10
	pSB1A2	medium constitutieve promoter			july 23
ampicilline

@@ Line 40: / Line 40: @@
 |-
 | {{iGempart|BBa_J23101}}||/||constitutive promotor||taken from plates || 13μl ||||aug 11
+|-
+| {{iGempart|BBa_K238013}}||/||Exact blue light promotor ||cut with EcoRI and SpeI || 26μl ||||aug 12
 |}