Team:KULeuven/Notebook/Blue Light Receptor

From 2009.igem.org

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==13 July==
 
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*YCGF Gene, natural available in [http://ecoliwiki.net/colipedia/index.php/ycgF:Gene 3 E. Coli strains]. Can use the promotor of Ycgf gene for expression
 
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*Probable strain K12
 
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*Promotor voor YCGF gene:
 
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**AACAATCCAGGGTAATGGGTGAGGCGAGAGTAAGACGGTAACAGACATATCTTCTTG TGTCTTTCTTTTAATACCAAAACATAACCGTTTCTTTACATTGATAAAAAATGGAAAAAG TTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTT TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATT CTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGC'''ATG''' (bron: [www.ncbi.nlm.nih.gov])
 
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==14 July==
 
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*Primer selection for the promotor [Done]
 
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**Send mail to Institut for Biologie-Mikrobiologie ([http://www.ncbi.nlm.nih.gov/pubmed/19240136 The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli]) [Done]
 
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==15 July==
 
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*Mail Institut fur Mikrobiologie der Westfalischen, Wilhelms-Universitat Munster for the plasmid [Done]
 
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==23 July==
 
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* Primers for the blue light receptor have arrived
 
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==24 July==
 
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* PCR on primers and tested on agarose gel
 
= Week 1: 6 July 2009 - 12 July 2009 =
= Week 1: 6 July 2009 - 12 July 2009 =

Revision as of 13:37, 27 July 2009

Contents

Week 1: 6 July 2009 - 12 July 2009

Week 2: 13 July 2009 - 19 July 2009

[edit] Monday

  • YcgF Gene, naturally available in 3 E. Coli strains. Can use the promotor of Ycgf gene for expression
  • Probable strain K12
  • Promotor voor YcgF gene:
    • AACAATCCAGGGTAATGGGTGAGGCGAGAGTAAGACGGTAACAGACATATCTTCTTG TGTCTTTCTTTTAATACCAAAACATAACCGTTTCTTTACATTGATAAAAAATGGAAAAAG TTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTT TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATT CTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG
      (source: [1])

[edit] Tuesday

[edit] Wednesday

  • Mail Institut fur Mikrobiologie der Westfalischen, Wilhelms-Universitat Munster for the plasmid [Done]

Week 3: 20 July 2009 - 26 July 2009

[edit] Thursday

  • Primers for the blue light receptor have arrived

[edit] Friday

  • PCR on promoter region in MC4100 E.coli colony using primers and tested on agarose gel

Week 4: 27 July 2009 - 2 August 2009

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