Team:KULeuven/Notebook/Blue Light Receptor

From 2009.igem.org

Revision as of 13:34, 27 July 2009 by Deepstar (Talk | contribs)

Contents

13 July

  • YCGF Gene, natural available in 3 E. Coli strains. Can use the promotor of Ycgf gene for expression
  • Probable strain K12
  • Promotor voor YCGF gene:
    • AACAATCCAGGGTAATGGGTGAGGCGAGAGTAAGACGGTAACAGACATATCTTCTTG TGTCTTTCTTTTAATACCAAAACATAACCGTTTCTTTACATTGATAAAAAATGGAAAAAG TTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTT TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATT CTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG (bron: [www.ncbi.nlm.nih.gov])

14 July

15 July

  • Mail Institut fur Mikrobiologie der Westfalischen, Wilhelms-Universitat Munster for the plasmid [Done]

23 July

  • Primers for the blue light receptor have arrived

24 July

  • PCR on primers and tested on agarose gel

Week 1: 6 July 2009 - 12 July 2009

Week 2: 13 July 2009 - 19 July 2009

[edit] Monday

  • YcgF Gene, naturally available in 3 E. Coli strains. Can use the promotor of Ycgf gene for expression
  • Probable strain K12
  • Promotor voor YcgF gene:
    • AACAATCCAGGGTAATGGGTGAGGCGAGAGTAAGACGGTAACAGACATATCTTCTTG TGTCTTTCTTTTAATACCAAAACATAACCGTTTCTTTACATTGATAAAAAATGGAAAAAG TTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTT TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATT CTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG
      (source: [1])

[edit] Tuesday

[edit] Wednesday

  • Mail Institut fur Mikrobiologie der Westfalischen, Wilhelms-Universitat Munster for the plasmid [Done]

Week 3: 20 July 2009 - 26 July 2009

[edit] Thursday

  • Primers for the blue light receptor have arrived

[edit] Friday

  • PCR on promoter region in MC4100 E.coli colony using primers and tested on agarose gel

Week 4: 27 July 2009 - 2 August 2009

Retrieved from "http://2009.igem.org/Team:KULeuven/Notebook/Blue_Light_Receptor"