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Team:KULeuven/Notebook/Vanillin Production
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*Primer selection for the promotor [Done] | *Primer selection for the promotor [Done] | ||
**Send mail to Institut for Biologie-Mikrobiologie ([http://www.ncbi.nlm.nih.gov/pubmed/19240136 The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli]) [Done] | **Send mail to Institut for Biologie-Mikrobiologie ([http://www.ncbi.nlm.nih.gov/pubmed/19240136 The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli]) [Done] | ||
| + | |||
| + | ==16 July== | ||
| + | *Focus on the last two parts in the synthesis of vanillin since that pathway has been proven | ||
| + | **Can always add additional 3 paths | ||
Revision as of 10:55, 24 July 2009
13 July
- Vanillin synthesis: DNA not yet complete/in library [1]
- Combination of 5 genes; 3 ervan zijn beschikbaar
- Edinburgh Vanilla production (Synthese stappen door Edinburgh igem 2007)
- Vanillin production using metabolically engineered Escherichia coli under non-growing conditions, Ferrulic accid --> Vanillin, 5k bp
- Combination of 5 genes; 3 ervan zijn beschikbaar
14 July
- Primer selection for the promotor [Done]
- Send mail to Institut for Biologie-Mikrobiologie (The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli) [Done]
16 July
- Focus on the last two parts in the synthesis of vanillin since that pathway has been proven
- Can always add additional 3 paths
