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Team:KULeuven/Notebook/Vanillin Receptor
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==22 July== | ==22 July== | ||
| - | The Vanillin Receptor is not usable, Madrid also tried to use and ran into all sorts of problems: | + | Received mail from Valencia: The Vanillin Receptor is not usable, Madrid also tried to use and ran into all sorts of problems: |
''Problems span from the binding of the periplasmic protein to the vanillin molecule, to the function of the fusion protein, to the activation of the ompR.'' | ''Problems span from the binding of the periplasmic protein to the vanillin molecule, to the function of the fusion protein, to the activation of the ompR.'' | ||
Revision as of 11:18, 24 July 2009
Contents |
14 July
- Check biobricks for Vanillin detection
15 July
- Mail University of Valencia because of inconsistent sequence in the parts registry [Done]
22 July
Received mail from Valencia: The Vanillin Receptor is not usable, Madrid also tried to use and ran into all sorts of problems: Problems span from the binding of the periplasmic protein to the vanillin molecule, to the function of the fusion protein, to the activation of the ompR.
There are two solutions to this problem:
- Construct a different vanillin receptor
- Using VirA/VirG, a phenol receptor in agrobacterium .
- Detect an alternative substance that has a linear relationship with the vanillin concentration
- Vanillin inhibits the Ahl receptor
- Synthesis of another signaling molecule that will be sensed
24 July
Vanillin Receptor
Possible receptor is the VirA/VirG system from Agrobacterium, the VirA protein will bind to phenolic compounds and induce transcription of vir genes by phosphorylation of VirG. protein ChvE results in a broader response to phenolic compounds. papers:
[1] S C Winans, Two-way chemical signaling in Agrobacterium-plant interactions, Microbiol Mol Biol Rev. 1992 March; 56(1): 12-31
[2] Wen-Tao Peng, Yong-Woog Lee, and Eugene W. Nester, The Phenolic Recognition Profiles of the Agrobacterium tumefaciens VirA Protein Are Broadened by a High Level of the Sugar Binding Protein ChvE, Journal of Bacteriology, November 1998, p. 5632-5638, Vol. 180, No. 21
[3]Yong-Woog Lee, Shouguang Jin, Woong-Seop Sim, Eugene W. Nester, The sensing of plant signal molecules by Agrobacterium: genetic evidence for direct recognition of phenolic inducers by the VirA protein, Gene, Volume 179, Issue 1, 1996, Pages 83-88, ISSN 0378-1119, DOI: 10.1016/S0378-1119(96)00328-9.
[4]Y W Lee, S Jin, W S Sim, and E W Nester,Genetic evidence for direct sensing of phenolic compounds by the VirA protein of Agrobacterium tumefaciens, Proc Natl Acad Sci U S A. 1995 December 19; 92(26): 12245–12249.
