Team:KULeuven/Project/Details

From 2009.igem.org

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The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription.  
The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription.  
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We designed the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K238002 BBa_K238002] part in such way that irradiation with a certain amount of blue light activates transcription of key-mRNA. To achieve this, we purified the promoter-region of E. coli MC4100. After mutating out possible restriction sites, the blr promot [http://partsregistry.org/wiki/index.php?title=Part:BBa_K238000 BBa_K238000] part was added to the registry.
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We designed the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K238002 BBa_K238002] part in such way that irradiation with a certain amount of blue light activates transcription of key-mRNA. To achieve this, we purified the promoter-region of E. coli MC4100. After mutating out possible restriction sites, the blr promot, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K238000 BBa_K238000] part was added to the registry.
== Vanillin receptor ==
== Vanillin receptor ==

Revision as of 12:00, 31 August 2009

Contents

Blue light receptor

Biologie blue light.png

The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. We designed the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K238002 BBa_K238002] part in such way that irradiation with a certain amount of blue light activates transcription of key-mRNA. To achieve this, we purified the promoter-region of E. coli MC4100. After mutating out possible restriction sites, the blr promot, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K238000 BBa_K238000] part was added to the registry.

Vanillin receptor

Biologie vanillin receptor.png

Vanillin production

Biologie vanillin synthesis.png
Vanillin production overview starting from Tyrosine.

Key antikey

Biologie blue light.png
Biologie antikey.png