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Team:Newcastle/Labwork/29 September 2009
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===Introduction=== | ===Introduction=== | ||
| - | + | * Two transformations need to be done today, one for psc-pMutin4 and another for kinA-pGFP-rrnB | |
| - | + | ||
| - | + | ||
<br> | <br> | ||
===Experiment procedure=== | ===Experiment procedure=== | ||
| - | ==== | + | ==== Transformation for E.coli ==== |
| - | + | * Followed Phil's protocal. | |
| - | + | ||
| - | + | ||
| + | ==== Digest the Mini Prep result for smtA:pMutin4 transformation==== | ||
| + | * This transformation was carried out by Matt and Arun. | ||
| + | * HindIII and BamHI were used for digestion. | ||
| + | dd H2O 7ul | ||
| + | 10X fast digest buffer 2ul | ||
| + | Fast BamHI 0.5ul | ||
| + | Fast HindIII 0.5ul | ||
| + | Mini Prep DNA 10ul | ||
| + | ------------------------------ | ||
| + | 20ul | ||
| + | * 37C 1 hour. | ||
| + | * Run the sample on 0.8% agarose gel. | ||
===Conclusion=== | ===Conclusion=== | ||
| - | + | * After the digestion of smtA:pMutin4 Mini result, the 12 strains are not the right clone. | |
<br> | <br> | ||
[[Image:|400px|center]] | [[Image:|400px|center]] | ||
| - | |||
| - | |||
Revision as of 00:25, 21 October 2009
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Formal Lab Session - 29th September 2009
Today we did miniprep for the ten ON cultures. Run them on thel gel.
After the results we did a restriction digest with HindIII and BamHI to find out the right colony.
However we did not get good results and we could not find the right colony we were looking for.
Chassis team
Introduction
- Two transformations need to be done today, one for psc-pMutin4 and another for kinA-pGFP-rrnB
Experiment procedure
Transformation for E.coli
- Followed Phil's protocal.
Digest the Mini Prep result for smtA:pMutin4 transformation
- This transformation was carried out by Matt and Arun.
- HindIII and BamHI were used for digestion.
dd H2O 7ul
10X fast digest buffer 2ul
Fast BamHI 0.5ul
Fast HindIII 0.5ul
Mini Prep DNA 10ul
------------------------------
20ul
- 37C 1 hour.
- Run the sample on 0.8% agarose gel.
Conclusion
- After the digestion of smtA:pMutin4 Mini result, the 12 strains are not the right clone.
[[Image:|400px|center]]
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