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Team:Newcastle/Labwork/8 October 2009
From 2009.igem.org
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== Experiment procedure == | == Experiment procedure == | ||
| - | + | ===pmutin4 digest === | |
| - | === pmutin4 digest with HindIII and BamHI | + | 1.pmutin4 digest with HindIII and BamHI |
H2O 26ul | H2O 26ul | ||
10X Buffer E 5ul | 10X Buffer E 5ul | ||
| Line 21: | Line 21: | ||
BamHI 1ul | BamHI 1ul | ||
| - | + | 2.pmutin4 control digest with HindIII | |
H2O 13.5 ul | H2O 13.5 ul | ||
10X Buffer E 1ul | 10X Buffer E 1ul | ||
| Line 28: | Line 28: | ||
HindIII 0.5ul | HindIII 0.5ul | ||
| - | + | 3.pmutin4 control digest with BamHI | |
H2O 13.5 ul | H2O 13.5 ul | ||
10X Buffer E 1ul | 10X Buffer E 1ul | ||
| Line 35: | Line 35: | ||
BamHI 0.5ul | BamHI 0.5ul | ||
| - | + | 4.pSB1AT3 control digest with HindIII and BamHI | |
H2O 13 ul | H2O 13 ul | ||
10X Buffer E 1ul | 10X Buffer E 1ul | ||
Revision as of 01:58, 21 October 2009
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Formal Lab Session - 8th October 2009
Introduction
- Today we did restriction digets again with pmutin4 with the new enzymes. We concluded that the enzymes we used may have been contaminated. We ordered normal new enzymes and used them instead.
- We also did PCR fragment digests (from the cleaned PCr product) with BamHI and HindIII
- We run the digestions on the gel and cut PCR fragment and pmutin4 from the gel to clean up.
Experiment procedure
pmutin4 digest
1.pmutin4 digest with HindIII and BamHI
H2O 26ul 10X Buffer E 5ul BSA 0.5 ul (diluted) DNA 15ul HindIII 1ul BamHI 1ul
2.pmutin4 control digest with HindIII
H2O 13.5 ul 10X Buffer E 1ul BSA 0.5 ul (diluted) DNA 3ul HindIII 0.5ul
3.pmutin4 control digest with BamHI
H2O 13.5 ul 10X Buffer E 1ul BSA 0.5 ul (diluted) DNA 3ul BamHI 0.5ul
4.pSB1AT3 control digest with HindIII and BamHI
H2O 13 ul 10X Buffer E 1ul BSA 0.5 ul (diluted) DNA 3ul HindIII 0.5ul BamHI 0.5ul
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News
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- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
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