Team:TUDelft/7 August 2009

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Did the rest of the assembly transformations. This time TSS and RubidiumCloride chemically competent cells were transformed with all ten constructs. LB medium was used instead of SOC, because all the SOC was used yesterday.  
Did the rest of the assembly transformations. This time TSS and RubidiumCloride chemically competent cells were transformed with all ten constructs. LB medium was used instead of SOC, because all the SOC was used yesterday.  
 +
 +
 +
===Calin===
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CA had colonies. But not all expressed GFP:
 +
 +
{| border="1" align="center"
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|- align="center"
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| Set || GFP colonies || Non-GFP colonies
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|- align="center"
 +
| RbCl ||  3 || 140
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|- align="center"
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| TSS ||  1? || 7
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|- align="center"
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| ElectroPoration ||  6 || 54
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|}
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 +
The long knockout primers and trbK gene arrived. Made a backup AMP plate for trbK. Rehydrated and electroporated BBa_J13002 (pTet-RBS).
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{| border="1" align="center"
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|- align="center"
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| Set || Time Constant
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|- align="center"
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| Assembly 1 || 4.4
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|- align="center"
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| BBa_J13002||  4.6
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|}
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 +
Daniel made CAM control plates at both high and low concentrations.
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 18:43, 7 August 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

7 August 2009

Tim Weenink

Did the rest of the assembly transformations. This time TSS and RubidiumCloride chemically competent cells were transformed with all ten constructs. LB medium was used instead of SOC, because all the SOC was used yesterday.


Calin

CA had colonies. But not all expressed GFP:

Set GFP colonies Non-GFP colonies
RbCl 3 140
TSS 1? 7
ElectroPoration 6 54

The long knockout primers and trbK gene arrived. Made a backup AMP plate for trbK. Rehydrated and electroporated BBa_J13002 (pTet-RBS).

Set Time Constant
Assembly 1 4.4
BBa_J13002 4.6

Daniel made CAM control plates at both high and low concentrations.

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