Team:Paris/1 September 2009
From 2009.igem.org
NoteBook
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Lab work
Verif purif
Products of:
PCR:
- FecI
- FecR (A) &(B) (B) Failed
- Tg3p Failed
digestion
- Plac rbs tetR
- TE3
- RBS
- TER
purified digestion
- pTet
- Cly A (N term & C term)
purification on gel
Products:
- FecI
- ClyA N term
- FecR (A)
- RBS
- TER
ligation with soufifi
Products:
- pFec (A) / P17
- TER/Plac rbs tetR
- RBS/YFP
- TE3/PSB1A3
- RFP/pTET
- RFP/TER