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Team:Paris/23 July 2009
From 2009.igem.org
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Brain work
- Modelling:
- Proteic binding force estimation for Tol/Pal system:
- use a simple estimation based on the existing values.(E_Prot=10-4O kJ/Mol*N_Prot)
- use a simple estimation based on the existing values of the OmpA familly.
- Docking values given by our own work on TolA/Pal.(aborted)
- Osmotic pressure estimation
- A mean on the wall cycle of the bacteria: 75 lb (bionumber estimation)
- Osmolarity of the periplasm:~300 mOsm
- Total model:
- Direct and simple shemtaics of the Model was designed: complet scheme begining of the choices for numerical values.
- Proteic binding force estimation for Tol/Pal system:
Sec-Tat Pathway:
- reading of 2 articles on tranlocation cinetics via Sec & Tat pathway
- the translocation rate of amino acids per time unit is constant on the Sec pathway
Ideas :
- Use a model of active diffusion across membrane
- Think about an evenemential simulation
To do list
Find the constrcution that are necessary for the fec system that Braun can't provide us with.
Modelling
- Get data on osmotic pressure.
- Find information on active diffusion across membranes.
- Find kinetics datas for complexation of tol-pal interaction as well as the translocation via tat and Sec export system.
- Get a better understanding of PMF.
- Test genomic DNA purification of S2
- More competent bacteria
