Team:Paris/27 August 2009
From 2009.igem.org
NoteBook
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Lab work
PCR on OmpA signal/g3p colony
Total volume = 25ul
2XSuper mix : 12,5 ul
Diluted bacteria : 5 ul
VF2 (10 uM) : 0,5 uL
VR (10uL) : 0,5 uL
H2O : 6,5 uL
gel purification
gel purif using promega kit
PCR
Pcr of:
- FecR part A & B
- Tg3p
purification on column
Purif of:
- OmpA linker (SacI/P) Failed (wrong size and two band)
- FecI (X/P) Failed (no DNA ?! )
- pBad (S/P) Failed (no DNA ?! )
Transfo
Jun Fos AIDA gen
Transfo
A40 TolRII: topo vector
O/N
A41 OmpAsignal : topovector