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Team:Paris/Freezer Primers
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iGEM > Paris > Freezer > Primers
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Primers
| [O1] Rev P1 | |||||
|---|---|---|---|---|---|
| Description | tolR | ||||
| Sequence (5' > 3') | TCTGCTGCAGCGGCCGCTACTAGTATTAGTAAGGCACATCTTTTGCGCCACCG | ||||
| Designer | D. Bikard | ||||
| Status | Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27) | ||||
| [O2] Fwd P2 (ERROR : Do not use) | |||||
|---|---|---|---|---|---|
| Description | tolR | ||||
| Sequence (5' > 3') | TCTGGAATTCGCGGCCGCTTCTAGATGAAATCAACATTGTACCGTTGCTGGACGTACTG | ||||
| Designer | D. Bikard | ||||
| Status | Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27) | ||||
| [O3] Rev P3 | |||||
|---|---|---|---|---|---|
| Description | tolA | ||||
| Sequence (5' > 3') | TCTGCTGCAGCGGCCGCTACTAGTATTATTACGGTTTGAAGTCCAATGGCG | ||||
| Designer | D. Bikard | ||||
| Status | Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27) | ||||
| [O4] Rev P4 (ERROR: Not designed for N-term fusion) | |||||
|---|---|---|---|---|---|
| Description | tolA | ||||
| Sequence (5' > 3') | TCTGGAATTCGCGGCCGCTTCTAGAATGGGTAATACTAAAAACAATGGCGC | ||||
| Designer | D. Bikard | ||||
| Status | Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27) | ||||
| [O5] Rev P5 | |||||
|---|---|---|---|---|---|
| Description | tatABC | ||||
| Sequence (5' > 3') | TCTGCTGCAGCGGCCGCTACTAGTATTATTATTCTTCAGTTTTTTCGCTTTCTGC | ||||
| Designer | D. Bikard | ||||
| Status | Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27) | ||||
| [06] Fwd P6 (ERROR: without RBS) | |||||
|---|---|---|---|---|---|
| Description | tatABC | ||||
| Sequence (5' > 3') | TCTGGAATTCGCGGCCGCTTCTAGAATGGGTGGTATCAGTATTTGG | ||||
| Designer | D. Bikard | ||||
| Status | Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27) | ||||
| [O7] Rev P7 (ERROR: not the good standard for fusion) | |||||
|---|---|---|---|---|---|
| Description | clyA fusion | ||||
| Sequence (5' > 3') | TTTCTGCAGCGGCCGCTACTAGTGACTTCAGGTACCTCAAAGAGTGTC | ||||
| Designer | D. Bikard | ||||
| Status | Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27) | ||||
| [O8] Fwd P8 (ERROR: No poly-Gly linker) | |||||
|---|---|---|---|---|---|
| Description | clyA fusion | ||||
| Sequence (5' > 3') | TTTGAATTCGCGGCCGCTTCTAGAATGACTGAAATCGTTGCAGATAAAA | ||||
| Designer | D. Bikard | ||||
| Status | Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27) | ||||
| [O9] clyA_Rw | |||||
|---|---|---|---|---|---|
| Description | clyA (for N term fusion) | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGACTTCAGGTACCTCAAAGAG | ||||
| Designer | G. Cambray | ||||
| Status | Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: no | ||||
| [O10] clyA_fusion_polyG_Rw | |||||
|---|---|---|---|---|---|
| Description | clyA fusion (with 5-Gly linker) | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTGCCGCCGCCGCCGCCGACTTCAGGTACCTCAAAGAG | ||||
| Designer | G. Cambray | ||||
| Status | Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
(08/05) | ||||
| [O11] ompA_signal_Fw | |||||
|---|---|---|---|---|---|
| Description | ompA signal for export to periplasm - from expression vector RBS as in pIN-III-ompA-2 vector - keeps vector original RBS | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAACGAGGGCAAAAAATGAAAAAG | ||||
| Designer | G. Cambray | ||||
| Status | Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
(08/05) | ||||
| [O12] ompA_signal_fusion_Rw | |||||
|---|---|---|---|---|---|
| Description | ompA signal for export to periplasm - from expression vector pIN-III-ompA-2 - original RBS from the vector | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCCTGCGCTACGGTAGCGAAAC | ||||
| Designer | G. Cambray | ||||
| Status | Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
(08/05) | ||||
| [O13] tolR_II_Fusion_Fw | |||||
|---|---|---|---|---|---|
| Description | For fusion of tolR domain II (standard 23) | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGGTCGATCTGCCAGACGCTAC | ||||
| Designer | G. Cambray | ||||
| Status | Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
(08/05) | ||||
| [O14] tatABC_RBS_Fw | |||||
|---|---|---|---|---|---|
| Description | Amplification from tatA - contains RBS | ||||
| Sequence (5' > 3') | TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGATGGGTGGTATCAGTATTTGG | ||||
| Designer | G. Cambray | ||||
| Status | Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
(08/05) | ||||
| [O15] TE3_fusion_Fw | |||||
|---|---|---|---|---|---|
| Description | Fusion of N-term domain of colicin E3 (standard 23) | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAAGCGGTGGCGATGGACGCGGCCATAAC | ||||
| Designer | C.Loy | ||||
| Status | Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
(08/05) | ||||
| [O16] TE3_Rw | |||||
|---|---|---|---|---|---|
| Description | Amplification of N-term domain of colicin E3 (standard 23) | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGCGTTCATAATTTCGCTCAG | ||||
| Designer | C.Loy | ||||
| Status | Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
(08/05) | ||||
| [O17] Tg3p_Rw | |||||
|---|---|---|---|---|---|
| Description | Amplification of N-term domain of M13's g3p | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAACACTGAGTTTCGTCACCAGTAC | ||||
| Designer | C. Loy | ||||
| Status | Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
(08/05) | ||||
| [O18] Tg3p_fusion_Fw | |||||
|---|---|---|---|---|---|
| Description | Fusion of N-term domain of M13's g3p (standard 23) | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAAAAAAATTATTATTCGCAATTCCTTTAG | ||||
| Designer | C.Loy | ||||
| Status | Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
(08/05) | ||||
| [O19] g3p_F | |||||
|---|---|---|---|---|---|
| Description | g3p | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTCCGCTGAAACTGTTGAAAGTTG | ||||
| Designer | G. Beauclair | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O20] g3p_R | |||||
|---|---|---|---|---|---|
| Description | g3p | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAGACTCCTTATTACGCAG | ||||
| Designer | G. Beauclair | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O21] Ompa_linker_F | |||||
|---|---|---|---|---|---|
| Description | For standardisation of OmpA linker (BBaK103006) | ||||
| Sequence (5' > 3') | TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGAAAGCTACTAAACTGGTACTGG | ||||
| Designer | G. Beauclair | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O22] Ompa_linker_R | |||||
|---|---|---|---|---|---|
| Description | For standardisation of OmpA linker (BBaK103006) | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGAGCTCCCTCCTCCAGAACC | ||||
| Designer | G. Beauclair | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O23] OmpAsignal-TE3_seq_Fw | |||||
|---|---|---|---|---|---|
| Description | For sequencing of part OmpAsignal-TE3 | ||||
| Sequence (5' > 3') | CACAAATAGCGAAAGATGAC | ||||
| Designer | C.Loy | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O24] OmpAsignal-TE3_seq_Rw | |||||
|---|---|---|---|---|---|
| Description | For sequencing of part OmpAsignal-TE3 | ||||
| Sequence (5' > 3') | ATCATCTGCGGGTAATGATG | ||||
| Designer | C.Loy | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O25] VAMP2_Seq_Fw | |||||
|---|---|---|---|---|---|
| Description | For sequencing of VAMP2 (snare) | ||||
| Sequence (5' > 3') | ACTCACTATAGGGAGACCACA | ||||
| Designer | S.Boumahdi | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O26] VAMP2_Seq_Rv | |||||
|---|---|---|---|---|---|
| Description | For sequencing of VAMP2 (snare) | ||||
| Sequence (5' > 3') | GGGCTTTGTTAGCAGCCGGAT | ||||
| Designer | S.Boumahdi | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O27] ClyA_seq_Fw | |||||
|---|---|---|---|---|---|
| Description | For sequencing of clyA after amplification | ||||
| Sequence (5' > 3') | GCAGCTATTTCCAGTCACAG | ||||
| Designer | V.Du | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O28] ClyA_seq_Rw | |||||
|---|---|---|---|---|---|
| Description | For sequencing of clyA after amplification | ||||
| Sequence (5' > 3') | CGCCCGCAGCAATAGAATAG | ||||
| Designer | V.Du | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O29] PFecA_Fw | |||||
|---|---|---|---|---|---|
| Description | For signal transduction | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACGCGGTACTGGATAAACATTTC | ||||
| Designer | R.Bodinier | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O30] PFecA_Rv | |||||
|---|---|---|---|---|---|
| Description | For signal transduction | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTTTGTTGTGTTCAGCTATGAGT | ||||
| Designer | R.Bodinier | ||||
| Status | Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no | ||||
| [O31] clyA_RBS_Fw | |||||
|---|---|---|---|---|---|
| Description | amplify clyA with RBS in the primer (std 10) | ||||
| Sequence (5' > 3') | TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGACTGAAATCGTTGCAGATAAAAC | ||||
| Designer | G. Cambray | ||||
| Status | Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
(08/05) | ||||
| [O32] clyA fusion_polyG_Fw | |||||
|---|---|---|---|---|---|
| Description | clyA fusion Fw (with 5-Gly linker) | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGCGGCGGCGGCGGCACTGAAATCGTTGCAGATAAAAC | ||||
| Designer | G. Cambray | ||||
| Status | Ordered: 07/27 ; Received: no ; Used in wet lab: no
( ) | ||||
