Team:Paris/Protocols transformation

From 2009.igem.org


Protocol for bacterial transformation

  • 250µl of DH5α competent bacteria (by RbCl)
  • Add 2µl of DNA (ex Biobrick)
  • Incubation during 20min on ice
  • Heat choc 45second at 42°C
  • Leave on ice 2min
  • Add 1ml of LB preheated at 42°C
  • Incubation 1 hour at 37°C under agitation
  • Put 100µl of culture under plate
  • Centrifuge 2min at 6rpm MAX for 2 min
  • Put 100µl of concentrated culture
  • Incubate overnight at 37°C