Team:Paris/Production testing delay
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== Delay == | == Delay == | ||
- | + | We separate the construction on two plasmids : | |
- | |||
+ | [[Team:Paris/Production_testing_delay#part1 | Part1 : testing delay phase 1]] | ||
+ | [[Team:Paris/Production_testing_delay#functional testing of the part 1 | Part1 : functional testing of the part 1]] | ||
- | |||
- | [[ | + | [[Team:Paris/Production_testing_delay#part2 | Part2 : testing delay phase 2]] |
+ | [[Team:Paris/Production_testing_delay#functional testing of the part 2 | Part2 : functional testing of the part 2]] | ||
+ | |||
+ | |||
+ | ===part1 === | ||
+ | [[Image:Paris_construc_3_delay1.jpg |800px|center|plasmid = PSB3T5]] | ||
Line 34: | Line 39: | ||
Experiments ran : | Experiments ran : | ||
+ | |||
{| | {| | ||
- | |- style="background: #0a3585; text-align: center; color: | + | |- style="background: #0a3585; text-align: center; color:black; font-weight:bold; " |
- | |width= 300px| | + | |width="300px" ; bgcolor="#F8F8F8"| column 1 |
- | |width=300px| | + | |width="300px" ; bgcolor="#E0E3FE"| column 2 |
- | |width=300px| | + | |width="300px" ; bgcolor="#CBD1FD"| column 3 |
+ | |width="300px" ; bgcolor="#BCCDFD"| column 4 | ||
+ | |||
|- style="background: #bebebe; text-align: center;" | |- style="background: #bebebe; text-align: center;" | ||
|'''PCR :''' | |'''PCR :''' | ||
- | + | TatABCE matrix : | |
- | + | ||
|'''PCR :''' | |'''PCR :''' | ||
|'''PCR :''' | |'''PCR :''' | ||
- | + | |'''PCR :''' | |
|- style="background: #d8d8d8; text-align: center;" | |- style="background: #d8d8d8; text-align: center;" | ||
Line 58: | Line 65: | ||
|'''Verification on gel :''' | |'''Verification on gel :''' | ||
- | + | - | |
|'''Verification on gel :''' | |'''Verification on gel :''' | ||
- | + | - | |
+ | |'''Verification on gel :''' | ||
+ | |||
+ | - | ||
|- style="background: #bebebe; text-align: center;" | |- style="background: #bebebe; text-align: center;" | ||
Line 71: | Line 81: | ||
|'''Purification on gel :''' | |'''Purification on gel :''' | ||
- | + | - | |
|'''Purification on gel :''' | |'''Purification on gel :''' | ||
- | + | - | |
+ | |'''Purification on gel :''' | ||
+ | |||
+ | - | ||
+ | |||
Line 81: | Line 95: | ||
|'''digestion:''' | |'''digestion:''' | ||
+ | TatABCE | ||
+ | |'''digestion:''' | ||
+ | |'''digestion:''' | ||
+ | |'''digestion:''' | ||
+ | |- style="background: #bebebe; text-align: center;" | ||
+ | |'''verification digestion:''' | ||
+ | ok | ||
+ | |'''verification digestion:''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |'''verification digestion:''' | ||
+ | |||
+ | - | ||
+ | |'''verification digestion:''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |||
+ | |- style="background: #d8d8d8; text-align: center;" | ||
+ | |'''ligation:''' | ||
+ | |||
+ | TatABCE | ||
+ | |||
+ | |'''ligation:''' | ||
+ | |'''ligation:''' | ||
+ | |'''ligation:''' | ||
+ | |||
+ | |- style="background: #bebebe; text-align: center;" | ||
+ | |'''PCR colo :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''PCR colo :''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |'''PCR colo :''' | ||
+ | |||
+ | - | ||
+ | |'''PCR colo :''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |- style="background: #d8d8d8; text-align: center;" | ||
+ | |'''miniprep:''' | ||
+ | |||
+ | STOPPED | ||
+ | |||
+ | |'''miniprep:''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |'''miniprep:''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |'''miniprep:''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |- style="background: #bebebe; text-align: center;" | ||
+ | |'''sequencing :''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |'''sequencing :''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |'''sequencing :''' | ||
+ | |||
+ | - | ||
+ | |'''sequencing :''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |} | ||
+ | |||
+ | ===functional testing of the part 1=== | ||
+ | |||
+ | PBAD LacI LVA- YFP was transforme into Top ten Bacteria and grew on medium : | ||
+ | |||
+ | |||
+ | 1% arabinose | ||
+ | |||
+ | 1% glucose | ||
+ | |||
+ | LB pure | ||
+ | |||
+ | |||
+ | <html> | ||
+ | </div> | ||
+ | <div id="paris_content_boxtop"> | ||
+ | </div> | ||
+ | <div id="paris_content"> | ||
+ | </html> | ||
+ | |||
+ | ===part2 === | ||
+ | |||
+ | [[Image:Paris_construc_5_delay2.jpg |800px|center|plasmid = ]] | ||
+ | |||
+ | |||
+ | |||
+ | Time required : A lOT !!!!! | ||
+ | |||
+ | Experiments ran : | ||
+ | |||
+ | {| | ||
+ | |- style="background: #0a3585; text-align: center; color:black; font-weight:bold; " | ||
+ | |width="300px" ; bgcolor="#F8F8F8"| column 1 | ||
+ | |width="300px" ; bgcolor="#E0E3FE"| column 2 | ||
+ | |width="300px" ; bgcolor="#CBD1FD"| column 3 | ||
+ | |width="300px" ; bgcolor="#BCCDFD"| column 4 | ||
+ | |||
+ | |||
+ | |- style="background: #bebebe; text-align: center;" | ||
+ | |'''PCR :''' | ||
+ | |||
+ | TatABCE matrix : | ||
+ | |||
+ | |'''PCR :''' | ||
+ | |'''PCR :''' | ||
+ | |'''PCR :''' | ||
+ | |||
+ | |- style="background: #d8d8d8; text-align: center;" | ||
+ | |'''Verification on gel :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''Verification on gel :''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |'''Verification on gel :''' | ||
+ | |||
+ | - | ||
+ | |'''Verification on gel :''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |- style="background: #bebebe; text-align: center;" | ||
+ | |'''Purification on gel :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''Purification on gel :''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |'''Purification on gel :''' | ||
+ | |||
+ | - | ||
+ | |'''Purification on gel :''' | ||
+ | |||
+ | - | ||
+ | |||
+ | |||
+ | |||
+ | |- style="background: #d8d8d8; text-align: center;" | ||
+ | |'''digestion:''' | ||
+ | |||
+ | TatABCE | ||
+ | |||
+ | |'''digestion:''' | ||
|'''digestion:''' | |'''digestion:''' | ||
|'''digestion:''' | |'''digestion:''' | ||
Line 95: | Line 274: | ||
|'''verification digestion:''' | |'''verification digestion:''' | ||
- | + | - | |
|'''verification digestion:''' | |'''verification digestion:''' | ||
- | + | - | |
+ | |'''verification digestion:''' | ||
+ | |||
+ | - | ||
Line 105: | Line 287: | ||
|'''ligation:''' | |'''ligation:''' | ||
- | + | TatABCE | |
|'''ligation:''' | |'''ligation:''' | ||
|'''ligation:''' | |'''ligation:''' | ||
- | + | |'''ligation:''' | |
|- style="background: #bebebe; text-align: center;" | |- style="background: #bebebe; text-align: center;" | ||
Line 118: | Line 300: | ||
|'''PCR colo :''' | |'''PCR colo :''' | ||
- | + | - | |
|'''PCR colo :''' | |'''PCR colo :''' | ||
- | + | - | |
+ | |'''PCR colo :''' | ||
+ | - | ||
|- style="background: #d8d8d8; text-align: center;" | |- style="background: #d8d8d8; text-align: center;" | ||
|'''miniprep:''' | |'''miniprep:''' | ||
- | + | STOPPED | |
|'''miniprep:''' | |'''miniprep:''' | ||
- | + | - | |
|'''miniprep:''' | |'''miniprep:''' | ||
- | + | - | |
+ | |'''miniprep:''' | ||
+ | |||
+ | - | ||
|- style="background: #bebebe; text-align: center;" | |- style="background: #bebebe; text-align: center;" | ||
|'''sequencing :''' | |'''sequencing :''' | ||
- | + | - | |
|'''sequencing :''' | |'''sequencing :''' | ||
- | + | - | |
|'''sequencing :''' | |'''sequencing :''' | ||
- | + | - | |
+ | |'''sequencing :''' | ||
+ | |||
+ | - | ||
|} | |} | ||
+ | |||
+ | ===functional testing of the part 2=== | ||
+ | |||
+ | Ptet RFP Plac TetR was tranforme into Top10 bacteria and grew on media : | ||
+ | |||
+ | |||
+ | 0,1% IPTG . | ||
+ | |||
+ | 1% glucose. | ||
+ | |||
+ | LB pure. | ||
+ | |||
+ | |||
+ | |||
+ | [[Team:Paris/Production_testing_delay#top | return to the top]] |
Latest revision as of 14:43, 18 October 2009
iGEM > Paris > Production > Testing > delay
Delay
We separate the construction on two plasmids :
Part1 : functional testing of the part 1
Part2 : functional testing of the part 2
part1
Time required : A lOT !!!!!
Experiments ran :
column 1 | column 2 | column 3 | column 4
|
PCR :
TatABCE matrix : | PCR : | PCR : | PCR : |
Verification on gel :
ok | Verification on gel :
- | Verification on gel :
- | Verification on gel :
- |
Purification on gel :
ok | Purification on gel :
- | Purification on gel :
- | Purification on gel :
-
|
digestion:
TatABCE | digestion: | digestion: | digestion: |
verification digestion:
ok | verification digestion:
- | verification digestion:
- | verification digestion:
-
|
ligation:
TatABCE | ligation: | ligation: | ligation: |
PCR colo :
ok | PCR colo :
- | PCR colo :
- | PCR colo :
- |
miniprep:
STOPPED | miniprep:
- | miniprep:
- | miniprep:
- |
sequencing :
- | sequencing :
- | sequencing :
- | sequencing :
- |
functional testing of the part 1
PBAD LacI LVA- YFP was transforme into Top ten Bacteria and grew on medium :
1% arabinose
1% glucose
LB pure
part2
Time required : A lOT !!!!!
Experiments ran :
column 1 | column 2 | column 3 | column 4
|
PCR :
TatABCE matrix : | PCR : | PCR : | PCR : |
Verification on gel :
ok | Verification on gel :
- | Verification on gel :
- | Verification on gel :
- |
Purification on gel :
ok | Purification on gel :
- | Purification on gel :
- | Purification on gel :
-
|
digestion:
TatABCE | digestion: | digestion: | digestion: |
verification digestion:
ok | verification digestion:
- | verification digestion:
- | verification digestion:
-
|
ligation:
TatABCE | ligation: | ligation: | ligation: |
PCR colo :
ok | PCR colo :
- | PCR colo :
- | PCR colo :
- |
miniprep:
STOPPED | miniprep:
- | miniprep:
- | miniprep:
- |
sequencing :
- | sequencing :
- | sequencing :
- | sequencing :
- |
functional testing of the part 2
Ptet RFP Plac TetR was tranforme into Top10 bacteria and grew on media :
0,1% IPTG .
1% glucose.
LB pure.