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Team:TUDelft/26 August 2009
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Restriction with E and S of PCR results for Locks and Keys. Besides restriction with the same enzymes of pSB1A3 backbone. Ligation of locks and keys in Amp backbones. Transformation and culture in plates and tubes. | Restriction with E and S of PCR results for Locks and Keys. Besides restriction with the same enzymes of pSB1A3 backbone. Ligation of locks and keys in Amp backbones. Transformation and culture in plates and tubes. | ||
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| + | ===Calin=== | ||
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| + | Got sequencing results back. CE sequence confirmed and CB partially confirmed. Other reactions did not work. | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 12:41, 19 October 2009
Lab Notebook
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26 August 2009
Orr
I helped Daniel making some plates, as well as made some more LB medium.
Saeed
8 colonies of the transformation !Z( 2 from each plate)were grown overnight for miniprep. The used colonies are named:
- !Za
- !Zb
- !Zc
- !Zd
- !Ze
- !Zf
- !Zg
- !Zh
Daniel
Restriction with E and S of PCR results for Locks and Keys. Besides restriction with the same enzymes of pSB1A3 backbone. Ligation of locks and keys in Amp backbones. Transformation and culture in plates and tubes.
Calin
Got sequencing results back. CE sequence confirmed and CB partially confirmed. Other reactions did not work.
