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Team:TUDelft/20 August 2009
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| - | Meeting with Groningen's team (see pictures) | + | Meeting with Groningen's team (see [https://2009.igem.org/Groningen_(and_Amsterdam)_visiting_Delft pictures]) |
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| + | ===Calin=== | ||
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| + | Checked KO plates. Some small colonies. Left plates in incubator. | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 12:55, 19 October 2009
Lab Notebook
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20 August 2009
Tim Weenink
We got colonies on yesterdays transformations. RbCl plates had about 20 colonies each, while Electrocompetent cells were hundreds of colonies. Finally an oldfashioned highly efficient transformation. I suspect transformations with larger parts (>1kb) work better.
I inoculated some of the colonies. These are the names of the samples:
R/E stands for RbCl/Electrocompetent (resp) 1C3 stands for the used backbone (pSB1C3) α ß stands for plate numbers 1,2 stands for colony numbers
- !SIIR1C3α1
- !SIIR1C3α2
- !SIIR1C3ß1
- !SIIR1C3ß2
- !SIIE1C3α1
- !SIIE1C3α2
- !SIIE1C3ß1
- !SIIE1C3ß2
Daniel
Growth in assemblies 1A, 2A, 3A, 4A and 5A. 6A failbut we found a biobrick with the same structure and we have it in plates already!!
Miniprep of succeed assemblies:
| Assembly | [DNA] ng/uL |
| 1A | 20.5 |
| 2A I | 18.7 |
| 2A II | 26.5 |
| 2A III | 25.5 |
| 2A IV | 32.2 |
| 3A | 13 |
| 4A I | 13.9 |
| 4A II | 8.7 |
| 5A | 4.9 |
Meeting with Groningen's team (see pictures)
Calin
Checked KO plates. Some small colonies. Left plates in incubator.
