Team:Paris/Freezer Digestion Products

From 2009.igem.org

(Difference between revisions)
(→Digestion Products)
(→Digestion Products)
 
(6 intermediate revisions not shown)
Line 1: Line 1:
 +
<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer_Digestion_Products#bottom | Digestion Products]]
{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
-
==Freezer==
 
-
<center>
+
== Digestion Products ==
-
[[Team:Paris/Freezer_Primers#Freezer | Primers]] -
+
<html>
-
[[Team:Paris/Freezer_PCR_products#Freezer | PCR products]] - 
+
<style type="text/css">
-
[[Team:Paris/Freezer_Plasmids#Freezer | Plasmids]] -
+
#left-side {
-
[[Team:Paris/Freezer_Digestion_Products#Freezer | Digestion Products]] -
+
    position: absolute;
-
[[Team:Paris/Freezer_Unexpected_digestion_results#Freezer | Unexpected digestion results]] -
+
    height: 23px;
-
[[Team:Paris/Freezer_Strains_(Glycerol stock)#Freezer | Strains (Glycerol stock)]] - [[Team:Paris/constructions#Freezer | Construction in progress]]
+
    width: 30px;
 +
    top: 0px;
 +
    left: 10px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
 +
    z-index:4;
 +
}
 +
 
 +
#middle-side {
 +
    height: 25px;
 +
    width: 660px;
 +
    position: absolute;
 +
    top: 0px;
 +
    left: 30px;
 +
    margin-top:10px;
 +
    padding-top: 5px;
 +
    background: #dadada;
 +
    z-index:5;
 +
}
 +
 
 +
#right-side {
 +
    position: absolute;
 +
    height: 23px;
 +
    width: 30px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    top: 0px;
 +
    left: 670px;
 +
    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
 +
    z-index:4;
 +
}
 +
 
 +
a.menu_sub {
 +
    padding-left: 7px;
 +
    padding-right: 7px;
 +
}
 +
 
 +
a.menu_sub_active {
 +
    padding-left: 7px;
 +
    padding-right: 7px;
 +
    color:#b0310e;
 +
    font-weight:bold;
 +
}
 +
</style>
 +
<div id="left-side"></div>
 +
<div id="middle-side"><center>
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
 +
<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
 +
<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
</center>
</center>
 +
</div>
 +
 +
<div id="right-side"></div>
 +
 +
</html>
 +
<br>
<br>
{|
{|
Line 26: Line 84:
|width=10% style="background: #FF0066;"| Chloranphenicol
|width=10% style="background: #FF0066;"| Chloranphenicol
|}
|}
-
<html>
 
-
</div>
 
-
<div id="paris_content_boxtop">
 
-
</div>
 
-
<div id="paris_content">
 
-
</html>
 
-
== Digestion Products ==
+
 
-
<div style="background-color:#FF0000; padding:1px; margin:1px; font-size:120%; text-align:center; font-weight:bold; border:1px solid black">Red = Vector</div>
+
-
<div style="background-color:#FFFF00; padding:1px; margin:1px; font-size:120%; text-align:center; font-weight:bold; border:1px solid black">Yellow = Insert</div>
+
{|
{|
Line 215: Line 265:
| Yes
| Yes
| ???
| ???
-
| [https://2009.igem.org/Team:Paris/11_August_2009 /11/08]
+
| [https://2009.igem.org/Team:Paris/11_August_2009 11/08]
 +
|- style="background: #ccccff; text-align: center;"
 +
|style="background:#FFFF00"| D16
 +
| pLac
 +
| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P21 P21]]BBa-I1014
 +
| P21
 +
| EcoRI
 +
| XbaI
 +
| ???
 +
| Yes
 +
| ???
 +
| [https://2009.igem.org/Team:Paris/11_August_2009 11/08]
|}
|}

Latest revision as of 15:26, 19 October 2009

iGEM > Paris > Freezer > Digestion Products


Digestion Products


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol


Digestion Products
Number Name Description Matrix ID Enz. 1 Enz. 2 Size (bp) Digested? Purified? [ng/µL]
D1 P3 [P3]BBa-J32015 XbaI PstI 66pb PelB leader sequence 47  ??? 08/04
D2 P4 [P4]BBa-B0014 XbaI PstI 95pb Double terminator bidirectionnal (B0012-B0011) 47  ??? 08/04
D3 P5 [P5]BBa-I14032 XbaI PstI 37pb pLac 47  ??? 08/04
D4 P7 [P7]Bba-R0040 XbaI PstI 54pb TetR repressible promote 47  ??? 08/04
D5 P8 [P8]BBa-I0500 XbaI PstI 1210pb pBad/araC 47  ??? 08/04
D6 P2 [P2] BBa-J61002 XbaI PstI 2053bp (and 895bp) Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). 47  ??? 08/04
D7 pSB2K3  ??? P15 XbaI PstI Size Yes Yes 08/07
D8 pSB1A3  ??? P16 XbaI PstI Size Yes Yes 08/07
D9 plbb RFP  ??? P17 EcoRI SpeI Size Yes Yes 08/07
D10 ptet  ??? P18 XbaI PstI Size Yes Yes 08/07
D11 RBS-tet  ??? P19 XbaI PstI Size Yes Yes 08/07
D12 pLac In order to open the vector to add RBS-tet (XbaI/PstI) P21 SpeI PstI Size Yes Yes 08/10
D13 OmpAsignal  ??? A11 XbaI PstI ~135 Yes Yes 11/07
D14 TolRII  ??? A13 XbaI PstI ~279 Yes Yes 11/07
D15 double terminator [P4]BBa-B0014 P4 EcoRI SpeI 95pb Yes  ??? 11/08
D16 pLac [P21]BBa-I1014 P21 EcoRI XbaI  ??? Yes  ??? 11/08

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_Digestion_Products"