Team:Paris/7 August 2009

From 2009.igem.org

Contents

NoteBook

June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

← Yesterday - Tomorrow →

August 7th

Lab work

Microscope

Microscopy

We try using the time laps with two filters

Molecular Biology

Reception of primers (oligos)

  • Primers from 08/03 arrived at Necker. References : O19 O20 O21 O22 O23 O24 O25 O26 O27 O28 O29 and O30.


  • All primers received by now are labeled and stored at -20°C in the "Oligos" box. Stock is 100µM. A 10µM solution (20µL) of each has also been prepared and is available in the "Oligos" box at -20°C. These 10µL solutions are to be used in priority.

Miniprep

P15, P16, P17, P18, P19

Digestion

P15, P16, P18, P19 by XbaI/PstI (Neb2), P17 by EcoRI/SpeI (Neb4)

Migration

0708 wiki digestion P18XP.png
0708 wiki digestion P18XP extract.png
0708 wiki digestion P15161719.png
0708 wiki digestion P15161719 extract.png

Purification

valeur

Verification sur gel

0708 wiki purif P19.png
0708 wiki purif P15161718.png

ON culture

P1, P2, P17, S31

Overnight PCR

compo

To do list

Matricule TODO
Luc microscope
Romain walking / wiki
Charlotte Jun/Fos system
Stoff Wiki update / Nair-tree protocol algorithm
Chris home sweet home ... but still looking for one
Lisa should be back
Caroline microscope
Souf
Vicard Lab
Pierre microscope / Modeling
Sylvain Run to Necker Hospital and come back with the primers. PCR
Guillaume lab


← Yesterday - Tomorrow →