Team:Paris

From 2009.igem.org

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===Abstract===
===Abstract===
'''Message in a Bubble: a robust inter-cellular communication system based on outer membrane vesicles.'''
'''Message in a Bubble: a robust inter-cellular communication system based on outer membrane vesicles.'''
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Sending a message across the ocean… Outer membrane vesicles (OMV), naturally produced by gram negative bacteria as E. Coli, are strong candidates for long-distance messaging. Our engineered communication platform consists of controlling OMV production by destabilizing membrane integrity through over-expression of specific periplasmic proteins (e.g., TolR). In order to control and modulate message content, we used fusions with OmpA signal sequence and the ClyA hemolysin as delivery tags. A targeting system was developed, based on the outer-membrane expression of Jun/Fos leucine zippers to control the vesicle flux between donor and recipient cells. Once received, the signal from incoming vesicles is transduced through a modified Fec pathway, whereby the receptor is provided by the OMV. Computational models provided insight to all of the above steps. Such reliable communications systems have wide biotechnological implications, ranging from targeted drugs delivery and detoxification to advanced division of labor or even cell-based computing.
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Sending a message across the ocean… Outer membrane vesicles (OMV), naturally produced by gram negative bacteria such as ''E. Coli'', are strong candidates for long-distance messaging. Our engineered communication platform consists in controlling OMV production by destabilizing membrane integrity through over-expression of specific periplasmic proteins (''e.g.'' TolR). In order to control and modulate message content, we used fusions with OmpA signal sequence and the ClyA hemolysin as delivery tags. A targeting system was developed, based on the outer-membrane expression of Jun/Fos leucine zippers to control the vesicle flux between donor and recipient cells. Once received, the signal from incoming vesicles is transduced through a modified Fec pathway, whereby the receptor is provided by the OMV. Computational models provided insight to all of the above steps. Such reliable communications systems have wide biotechnological implications, ranging from targeted drugs delivery and detoxification to advanced division of labor or even cell-based computing.
===Strategy===
===Strategy===
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===Navigation===
===Navigation===
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Here we are ! You have some problems to navigate in our wiki ?? Just look at [[Team:Paris/navigation#top | this page]], and we hope it will help you.
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<font color=red>flefevre : je propose qq lignes ici pour aider à la navigation: comment utilise t on votre wiki, liens importants, l'icone book pour la lecture dirigée </font>
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And, if your are interested in a guided visit of the iGEM Paris 2009 wiki,..., just open the book...
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=== iGEM Paris Team ===
=== iGEM Paris Team ===
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Hello everyone! welcome to our wiki. It's the 3rd year for the Paris Team and we're still motivated to do our best. This year we have a team of 13 students (what a luck). We are 6 biologists, 2 mathematicians, 1 computer scientist, 1 sociologist, 1 infectiologist, 1 geneticist, and 1 chemist. We've got the best of the best of the best supervisors like Ariel Lindner, Guillaume Cambray and Samuel Bottani. So... let's the contest begin! (Details on our team [[Team:Paris/Team#top | here]]).<br>
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'''Hello everyone and welcome on our wiki webpage !'''
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Additional information on all iGEM Paris team here [[http://www.igem-paris.org http://www.igem-paris.org]]<br>
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<font color=red>Page de presentation à revoir: plus positive et pas nécessairement liée aux années précédentes. Plus positive : nous sommes <i>encore</i> motivées... Encore heureux! Soyez factuels sur cette année. </font>
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We are a team of 13 highly motivated students ready to give our best in the iGEM competition. We decided to involve our different backgrounds (6 biologists, 2 mathematicians, a computer scientist, a sociologist, an infectiologist, a geneticist, and a chemist) and our enthusiasm in this scientific and human adventure.
 +
Regarding the team's supervisors, we have the honnor to have been adviced by some great scientists/professors like Ariel Lindner, Guillaume Cambray and Samuel Bottani among others, almost always at the [http://www.cri-paris.org CRI].<br>
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More details on our team [[Team:Paris/Team#top | here]].<br>
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More details on the CRI at the bottom of our [[Team:Paris/Acknowledgements#top | Acknowledgement page]].<br>
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Additional information about all iGEM Paris teams since 2007 here : [http://www.igem-paris.org http://www.igem-paris.org]<br>
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=== Our Sponsors ===
=== Our Sponsors ===
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<font color=red>Important pour ménager les suceptibilités que la taille des logosdes sponsors soit en rapport avec le niveau de leur aide... </font><br>
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<font color=red>flefevre: mettre echosens/biolabs dans un unique carré et mettre fondation bettencourt dans 2 carré, rajouter peut etre le mot : notre sponsor officiel et hisotrique </font>
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=== Collaborations and  Visitors  ===
=== Collaborations and  Visitors  ===
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With [https://2009.igem.org/Team:BCCS-Bristol  BCCS-Bristol] , we shared our modeling part concerning the production of vesicles which is supposed to be complementary of their and we hope that we will be able to combine the two approach to make one big model of the production , adressing , and fusion of the vesicles.  
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With the [https://2009.igem.org/Team:BCCS-Bristol  team BCCS-Bristol] , we shared our modeling part concerning the production of vesicles which is supposed to be complementary of their and we hope that we will be able to combine both approaches to make one big model of the production, adressing, and fusion of the vesicles. For more details, see our [[Team:Paris/Collaborations#top | Collaborations]] web page.
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For more details, see our [[Team:Paris/Collaborations#top | Collaborations]] web page.
 
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We have also created a poll concerning the Iphone Software and we sent it to the other iGEM teams, among which four replied. Find the results and more details on our [[Team:Paris/Collaborations#top | Collaborations]] page.
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We have also created a poll concerning the Iphone Software and we sent it to the other Igem team , 4 of them replied,
 
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Find  the results and more details on [[Team:Paris/Collaborations#top | Collaborations]] page.
 
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All members of our team answered to the Valencia ethics poll that is why you can see this gorgeous gold medal with our name on it. More seriously it was very interesting to see what the members answered to each question. Looking for more details ? see our [[Team:Paris/Collaborations#top | Collaborations]] web page.
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All the team answered to the Valencia ethics poll that is why you can see this gorgeous gold medal with our name on it. More seriously it was very interesting to see what all members answered to each questions.
 
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Looking for more details ? see our [[Team:Paris/Collaborations#top | Collaborations]] web page.
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And we thank all our visitors, without them we would not have such an eclectic cluster map !
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And we thank all the visitors, without them we couldn't have a so eclectic cluster map.
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<a href="http://www3.clustrmaps.com/counter/maps.php?url=https://2009.igem.org/Team:Paris" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=https://2009.igem.org/Team:Paris" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';" />
<a href="http://www3.clustrmaps.com/counter/maps.php?url=https://2009.igem.org/Team:Paris" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=https://2009.igem.org/Team:Paris" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';" />
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<a href="https://2009.igem.org/Team:Valencia/Human" target="_blank"> <img src="https://static.igem.org/mediawiki/2009/5/50/V_Paris.JPG" width="110" height="110"></a> Thank you <a href="https://2009.igem.org/Team:Valencia"><a3>Valencia</a3></a> !
<a href="https://2009.igem.org/Team:Valencia/Human" target="_blank"> <img src="https://static.igem.org/mediawiki/2009/5/50/V_Paris.JPG" width="110" height="110"></a> Thank you <a href="https://2009.igem.org/Team:Valencia"><a3>Valencia</a3></a> !
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=== Internal Notes ===
 
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<font color=red> Image très jolie mais qui nécessite une légente  pour explique les trois élements: production, vésicule, cible. Peut-être d'ailleurs ici mettre des liens directs vers les parties correspondantes du wiki.</font>
 
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<font color=red>Un élément à introduire dans le wiki et sur lequel je reviendrai de cesse tant qu'il ne sera pas là:  des liens de navigation pour une visite guidée, séquentielle du wiki. Qu'il puisse être possible de le parcourir facilement dans un ordre logique suivant votre histoire. Il est trop facile dans les wiikis de se perdre dans les ramifications des branches des différentes pages et de ne ressortir qu'avec une idée confuse du contenu du site.</font>
 
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<font color=red> Les menus de gauche ne sont pas clairs ainsi que l'architecture du site: de grandes parties mélangées à des sous parties: Adressing, Production, Reception ne devraient pas être au même niveau que Home, The Project, Ehics, Tools...
 
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En parcourant l'ensemble je pense que ce serait utile pour la vulgarisation du projet d'avoir une partie "Cell Biology Reminder" qui rappelerait les bases scientifiques nécessaire aiu projet: Gram-negative bacteria, The membrane of a gram-negative bacteria, Veiscles etc...
 
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<font color=red> flefevre : j'ai viré les parties non essentielles dans la page [[Team:Paris/Draft | Draft]]. On peut donc y retrouver les éléments précédemment écrits.
 
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<font color=red>flefevre : je pense qu il faut une page dedie a expliquer comment naviguer dans votre wiki, eventuellement une capture d une page qui explicite les differents menus.
 
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il y a en effet : <br>
 
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<font color=red>flefevre : je pense qu il faut augmenter la taille en largeur du menu de gauche pour pouvoir avoir des titres de menu long = 2 mots qui veulent dire quelques choses
 
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=== A FAIRE ===
 
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Project Overview  = Corriger
 
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Modelling Overview = A faire partie Ch
 
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Conclusion = A faire
 
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Export = Tat vs Sec : schéma
 
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Stratégie = relire
 
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Construction  = A faire
 
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ClyA = relire + stratégie : relire +
 
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Wet lab  = a finir
 
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Vesicule prod system = traduire; construction = à corriger
 
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Vesicule prod model = corriger
 
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Modeling vesicule creation = cf Peter
 
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Wet lab = pbme de mise en page
 
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Jun/Foss = NADA
 
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Snares : NADA
 
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Strategy + Construction = NADA
 
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Tansduction = a corriger
 
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Fec Simu = cf ch
 
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Wetlab : pbme mise en page
 

Latest revision as of 03:46, 22 October 2009

iGEM > Paris > Home > Synopsis

Contents

Message in a Bubble


Interactive schema

Abstract

Message in a Bubble: a robust inter-cellular communication system based on outer membrane vesicles. Sending a message across the ocean… Outer membrane vesicles (OMV), naturally produced by gram negative bacteria such as E. Coli, are strong candidates for long-distance messaging. Our engineered communication platform consists in controlling OMV production by destabilizing membrane integrity through over-expression of specific periplasmic proteins (e.g. TolR). In order to control and modulate message content, we used fusions with OmpA signal sequence and the ClyA hemolysin as delivery tags. A targeting system was developed, based on the outer-membrane expression of Jun/Fos leucine zippers to control the vesicle flux between donor and recipient cells. Once received, the signal from incoming vesicles is transduced through a modified Fec pathway, whereby the receptor is provided by the OMV. Computational models provided insight to all of the above steps. Such reliable communications systems have wide biotechnological implications, ranging from targeted drugs delivery and detoxification to advanced division of labor or even cell-based computing.

Strategy

We aimed developing a long distance communication system between gram-negative bacteria that is based on the ability of these organisms to produce Outer-Membrane Vesicles (OMVs). We designed a framework which can be easily expanded to a lot of different inputs/ouputs. We hope this standardized approach will increase our capacity to manipulate information and/or exchange it between bacteria. This can be used in any engineered biological process requiring transformation or a system of information transfer, including medical applications and bio-remediation.


We did our best to standardize:

  1. the increase of vesicle production;
  2. the system to address proteins to these vesicles;
  3. their fusion with target bacteria.

You can find detailed information in our OMV Project description or in our different parts.


By analogy to the Internet Protocol, we called our process a Bacterial Protocol. We found that the title Message in a bubble described it quite nicely. In a simple way, the comprehension of vesicles process sounds like a build of a bacteria language.


For more information on how we came up with this idea, please have a look at our brainstorming area.

Navigation

Here we are ! You have some problems to navigate in our wiki ?? Just look at this page, and we hope it will help you.

And, if your are interested in a guided visit of the iGEM Paris 2009 wiki,..., just open the book...

Open book.gif

← Previous - Next →

Extended Team

iGEM Paris Team

Hello everyone and welcome on our wiki webpage !


We are a team of 13 highly motivated students ready to give our best in the iGEM competition. We decided to involve our different backgrounds (6 biologists, 2 mathematicians, a computer scientist, a sociologist, an infectiologist, a geneticist, and a chemist) and our enthusiasm in this scientific and human adventure. Regarding the team's supervisors, we have the honnor to have been adviced by some great scientists/professors like Ariel Lindner, Guillaume Cambray and Samuel Bottani among others, almost always at the [http://www.cri-paris.org CRI].


More details on our team here.
More details on the CRI at the bottom of our Acknowledgement page.
Additional information about all iGEM Paris teams since 2007 here : [http://www.igem-paris.org http://www.igem-paris.org]


Our Sponsors

Collaborations and Visitors

With the team BCCS-Bristol , we shared our modeling part concerning the production of vesicles which is supposed to be complementary of their and we hope that we will be able to combine both approaches to make one big model of the production, adressing, and fusion of the vesicles. For more details, see our Collaborations web page.


We have also created a poll concerning the Iphone Software and we sent it to the other iGEM teams, among which four replied. Find the results and more details on our Collaborations page.


All members of our team answered to the Valencia ethics poll that is why you can see this gorgeous gold medal with our name on it. More seriously it was very interesting to see what the members answered to each question. Looking for more details ? see our Collaborations web page.


And we thank all our visitors, without them we would not have such an eclectic cluster map !


Locations of visitors to this page Thank you Valencia !

Retrieved from "http://2009.igem.org/Team:Paris"