Team:TUDelft/30 July 2009

From 2009.igem.org

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15:20 Plated assemblies
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15:40 inoculated (2*4) *S and *T cultures
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16:55 Inoculated 2 !A cultures
===Calin===
===Calin===

Revision as of 15:12, 30 July 2009

Lab Notebook

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30 July 2009

Tim Weenink

prepared assembly mixes for !B, !C and !D constructs. Standard Ginko assembly kit was used. DNA concentrations as below (these mixtures were added to 7.5 µl of digestion mixture):

Assembly name position Components volume in µl
 !B Upstream  !A DNA 33.0
H20 9.5
Downstream BBa_B0015 DNA 10.0
H20 32.5
Backbone pSB1AC3 DNA 6.5
H20 36
 !C Upstream BBa_R0040 DNA 10.0
H20 32.5
Downstream *I6 DNA 5.5
H20 37.0
Backbone pSB1AC3 DNA 6.5
H20 36
 !D Upstream *I6 DNA 5.5
H20 37.0
Downstream BBa_K145201 DNA 18.0
H20 24.5
Backbone pSB1AC3 DNA 6.5
H20 36


15:20 Plated assemblies


15:40 inoculated (2*4) *S and *T cultures

16:55 Inoculated 2 !A cultures

Calin

Plated three backbones left over from the transformation the other day. Did a digest on oriT-R with E and P.


Tim Vos

Minipreped pSB4C5, pSB.


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