Team:Paris/27 July 2009

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June
M	T	W	T	F	S	S
[http://2009.igem.org/Team:Paris/1_June_2009 1]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/2_June_2009&action=edit 2]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/3_June_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/4_June_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/5_June_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/6_June_2009&action=edit 6]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/8_June_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/9_June_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/10_June_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/11_June_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/12_June_2009&action=edit 12]	[http://2009.igem.org/Team:Paris/13_June_2009 13]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/14_June_2009&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/15_June_2009&action=edit 15]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/16_June_2009&action=edit 16]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/17_June_2009&action=edit 17]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/18_June_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/19_June_2009&action=edit 19]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/20_June_2009&action=edit 20]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/22_June_2009&action=edit 22]	[http://2009.igem.org/Team:Paris/23_June_2009 23]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/24_June_2009&action=edit 24]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/25_June_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/26_June_2009&action=edit 26]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/27_June_2009&action=edit 27]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/28_June_2009&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/29_June_2009&action=edit 29]	[http://2009.igem.org/Team:Paris/30_June_2009 30]

July
M	T	W	T	F	S	S
		[http://2009.igem.org/wiki/index.php?title=Team:Paris/1_July_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/2_July_2009&action=edit 2]	[http://2009.igem.org/Team:Paris/3_July_2009 3]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/4_July_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/6_July_2009&action=edit 6]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/7_July_2009&action=edit 7]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/8_July_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/9_July_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/10_July_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/11_July_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Team:Paris/12_July_2009&action=edit 12]
[http://2009.igem.org/Team:Paris/13_July_2009 13]	[http://2009.igem.org/Team:Paris/14_July_2009 14]	[http://2009.igem.org/Team:Paris/15_July_2009 15]	[http://2009.igem.org/Team:Paris/16_July_2009 16]	[http://2009.igem.org/Team:Paris/17_July_2009 17]	[http://2009.igem.org/Team:Paris/18_July_2009 18]	[http://2009.igem.org/Team:Paris/19_July_2009 19]
[http://2009.igem.org/Team:Paris/20_July_2009 20]	[http://2009.igem.org/Team:Paris/21_July_2009 21]	[http://2009.igem.org/Team:Paris/22_July_2009 22]	[http://2009.igem.org/Team:Paris/23_July_2009 23]	[http://2009.igem.org/Team:Paris/24_July_2009 24]	[http://2009.igem.org/Team:Paris/25_July_2009 25]	[http://2009.igem.org/Team:Paris/26_July_2009 26]
[http://2009.igem.org/Team:Paris/27_July_2009 27]	[http://2009.igem.org/Team:Paris/28_July_2009 28]	[http://2009.igem.org/Team:Paris/29_July_2009 29]	[http://2009.igem.org/Team:Paris/30_July_2009 30]	[http://2009.igem.org/Team:Paris/31_July_2009 31]

August
M	T	W	T	F	S	S
					[http://2009.igem.org/Team:Paris/1_August_2009 1]	[http://2009.igem.org/Team:Paris/2_August_2009 2]
[http://2009.igem.org/Team:Paris/3_August_2009 3]	[http://2009.igem.org/Team:Paris/4_August_2009 4]	[http://2009.igem.org/Team:Paris/5_August_2009 5]	[http://2009.igem.org/Team:Paris/6_August_2009 6]	[http://2009.igem.org/Team:Paris/7_August_2009 7]	[http://2009.igem.org/Team:Paris/8_August_2009 8]	[http://2009.igem.org/Team:Paris/9_August_2009 9]
[http://2009.igem.org/Team:Paris/10_August_2009 10]	[http://2009.igem.org/Team:Paris/11_August_2009 11]	[http://2009.igem.org/Team:Paris/12_August_2009 12]	[http://2009.igem.org/Team:Paris/13_August_2009 13]	[http://2009.igem.org/Team:Paris/14_August_2009 14]	[http://2009.igem.org/Team:Paris/15_August_2009 15]	[http://2009.igem.org/Team:Paris/16_August_2009 16]
[http://2009.igem.org/Team:Paris/17_August_2009 17]	[http://2009.igem.org/Team:Paris/18_August_2009 18]	[http://2009.igem.org/Team:Paris/19_August_2009 19]	[http://2009.igem.org/Team:Paris/20_August_2009 20]	[http://2009.igem.org/Team:Paris/21_August_2009 21]	[http://2009.igem.org/Team:Paris/22_August_2009 22]	[http://2009.igem.org/Team:Paris/23_August_2009 23]
[http://2009.igem.org/Team:Paris/24_August_2009 24]	[http://2009.igem.org/Team:Paris/25_August_2009 25]	[http://2009.igem.org/Team:Paris/26_August_2009 26]	[http://2009.igem.org/Team:Paris/27_August_2009 27]	[http://2009.igem.org/Team:Paris/28_August_2009 28]	[http://2009.igem.org/Team:Paris/29_August_2009 29]	[http://2009.igem.org/Team:Paris/30_August_2009 30]
[http://2009.igem.org/Team:Paris/31_August_2009 31]

September
M	T	W	T	F	S	S
	[http://2009.igem.org/Team:Paris/1_September_2009 1]	[http://2009.igem.org/Team:Paris/2_September_2009 2]	[http://2009.igem.org/Team:Paris/3_September_2009 3]	[http://2009.igem.org/Team:Paris/4_September_2009 4]	[http://2009.igem.org/Team:Paris/5_September_2009 5]	[http://2009.igem.org/Team:Paris/6_September_2009 6]
[http://2009.igem.org/Team:Paris/7_September_2009 7]	[http://2009.igem.org/Team:Paris/8_September_2009 8]	[http://2009.igem.org/Team:Paris/9_September_2009 9]	[http://2009.igem.org/Team:Paris/10_September_2009 10]	[http://2009.igem.org/Team:Paris/11_September_2009 11]	[http://2009.igem.org/Team:Paris/12_September_2009 12]	[http://2009.igem.org/Team:Paris/13_September_2009 13]
[http://2009.igem.org/Team:Paris/14_September_2009 14]	[http://2009.igem.org/Team:Paris/15_September_2009 15]	[http://2009.igem.org/Team:Paris/16_September_2009 16]	[http://2009.igem.org/Team:Paris/17_September_2009 17]	[http://2009.igem.org/Team:Paris/18_September_2009 18]	[http://2009.igem.org/Team:Paris/19_September_2009 19]	[http://2009.igem.org/Team:Paris/20_September_2009 20]
[http://2009.igem.org/Team:Paris/21_September_2009 21]	[http://2009.igem.org/Team:Paris/22_September_2009 22]	[http://2009.igem.org/Team:Paris/23_September_2009 23]	[http://2009.igem.org/Team:Paris/24_September_2009 24]	[http://2009.igem.org/Team:Paris/25_September_2009 25]	[http://2009.igem.org/Team:Paris/26_September_2009 26]	[http://2009.igem.org/Team:Paris/27_September_2009 27]
[http://2009.igem.org/Team:Paris/28_September_2009 28]	[http://2009.igem.org/Team:Paris/29_September_2009 29]	[http://2009.igem.org/Team:Paris/30_September_2009 30]

October
M	T	W	T	F	S	S
			[http://2009.igem.org/Team:Paris/1_October_2009 1]	[http://2009.igem.org/Team:Paris/2_October_2009 2]	[http://2009.igem.org/Team:Paris/3_October_2009 3]	[http://2009.igem.org/Team:Paris/4_October_2009 4]
[http://2009.igem.org/Team:Paris/5_October_2009 5]	[http://2009.igem.org/Team:Paris/6_October_2009 6]	[http://2009.igem.org/Team:Paris/7_October_2009 7]	[http://2009.igem.org/Team:Paris/8_October_2009 8]	[http://2009.igem.org/Team:Paris/9_October_2009 9]	[http://2009.igem.org/Team:Paris/10_October_2009 10]	[http://2009.igem.org/Team:Paris/11_October_2009 11]
[http://2009.igem.org/Team:Paris/12_October_2009 12]	[http://2009.igem.org/Team:Paris/13_October_2009 13]	[http://2009.igem.org/Team:Paris/14_October_2009 14]	[http://2009.igem.org/Team:Paris/15_October_2009 15]	[http://2009.igem.org/Team:Paris/16_October_2009 16]	[http://2009.igem.org/Team:Paris/17_October_2009 17]	[http://2009.igem.org/Team:Paris/18_October_2009 18]
[http://2009.igem.org/Team:Paris/19_October_2009 19]	[http://2009.igem.org/Team:Paris/20_October_2009 20]	[http://2009.igem.org/Team:Paris/21_October_2009 21]	[http://2009.igem.org/Team:Paris/22_October_2009 22]	[http://2009.igem.org/Team:Paris/23_October_2009 23]	[http://2009.igem.org/Team:Paris/24_October_2009 24]	[http://2009.igem.org/Team:Paris/25_October_2009 25]
[http://2009.igem.org/Team:Paris/26_October_2009 26]	[http://2009.igem.org/Team:Paris/27_October_2009 27]	[http://2009.igem.org/Team:Paris/28_October_2009 28]	[http://2009.igem.org/Team:Paris/29_October_2009 29]	[http://2009.igem.org/Team:Paris/30_October_2009 30]	[http://2009.igem.org/Team:Paris/31_October_2009 31]

← Yesterday - Tomorrow →

July 27th

Brain work

edit please ^^

Lab work

Media: 15 LB agarosis ampiciline dishes were made.

PCR for oligo P1-P2 (tolR); P3-P4 (tolA); P5-P6 (tatABC); P7-P8 (clyA fusion). The protocol is stored under the name PCR quick load Taq mix:

Preparation for Vf=50µL:

-1µL Matrice

-14µL H2O

-5µ Oligo Fw (à 0,2M)

-5µL Oligo Rw (à 0,2M)

-25µL Tampon+Taq (Taq quick load master mix 2X)

PCR launched with a Tm at 60°C (programme quick load)

Buffer for Competent bacteria by RbCl
- [F9] Buffer I (250ml)
- [F10] Buffer II (125ml)

DH5 alpha competent bacteria by RbCl [Protocol]

To do list

migration of the PCR product on a BET gel.

@@ Line 30: / Line 30: @@
 ==Lab work==
-<u>media : </u>
+*Media: 15 LB agarosis ampiciline dishes were made.
-LB agarosis ampiciline dishes were made.
+<br>
+*PCR for oligo P1-P2 (tolR); P3-P4 (tolA); P5-P6 (tatABC); P7-P8 (clyA fusion).[[Team:Paris/Protocols_PCRqload | The protocol is stored under the name PCR quick load Taq mix:]]<br>
-<u>PCR :</u>
-<p> P1-P2=1; P3-P4=3; P5-P6=5; P7-P8=7</p><br>
-[[Team:Paris/Protocols_PCRqload | the protocol is stored under the name PCR quick load Taq mix :]]<br>