Team:TUDelft/31 July 2009

From 2009.igem.org

(Difference between revisions)
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| N/A || !A || 171.2 || 1.98 || 2.11
| N/A || !A || 171.2 || 1.98 || 2.11
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===Calin===
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Cultures in all pSB4C5 tubes. Did miniprep on all tubes and also made a plate.
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{| border="1" align="center"
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| Part || Concentration ng/µl || 260/280 || 260/230
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|- align="center"
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| pSB4C5 I || 123.0 || 1.91 || 2.06
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|- align="center"
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| pSB4C5 II || 125.8 || 1.92 || 2.29
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|- align="center"
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| pSB4C5 III || 130.7 || 1.95 || 2.07
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|}
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Todays gel confirmed that oriT-R digestion failed and pSB4C5 is way too large.
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[[Image:Gel31072009.png|650px]]
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{| border="1" align="center"
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| Well || Part || Expected Plasmid Size || Status
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| 22 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
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| 23 || oriT E + P digest || 2079  || <font color=red>&#10006;</font>
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| 24 || pSB4C5 I || 3896  ||  <font color=red>&#10006;</font>
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|- align="center"
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| 25 || pSB4C5 II || 3896  ||  <font color=red>&#10006;</font>
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|- align="center"
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| 26 || pSB4C5 III || 3896  ||  <font color=red>&#10006;</font>
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|}
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R751 cells arrived. Made two plates without antibiotics.
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Digestions were started:
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 18:22, 31 July 2009

Lab Notebook

July
MTWTFSS
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20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
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3 4 5 6 7 8 9
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17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
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7 8 9 10 11 12 13
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21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

31 July 2009

Tim Weenink

Did some minipreps today. Then nanodropped them:

Biobrick name Internal reference Concentration ng/µl 260/280 260/230
BBa_K142205 *S2 86.4 2.01 2.03
BBa_K142205 *S3 107.7 2.03 2.11
BBa_K142205 *S4 69.0 1.97 1.96
BBa_K142205 *S5 87.4 1.98 1.99
BBa_K142202 *T1 128.5 1.99 2.09
BBa_K142202 *T2 129.4 2.02 2.16
BBa_K142202 *T3 126.6 1.97 2.06
BBa_K142202 *T4 106.7 1.95 2.05
N/A  !A 171.2 1.98 2.11

Calin

Cultures in all pSB4C5 tubes. Did miniprep on all tubes and also made a plate.

Part Concentration ng/µl 260/280 260/230
pSB4C5 I 123.0 1.91 2.06
pSB4C5 II 125.8 1.92 2.29
pSB4C5 III 130.7 1.95 2.07

Todays gel confirmed that oriT-R digestion failed and pSB4C5 is way too large.

Gel31072009.png


Well Part Expected Plasmid Size Status
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
23 oriT E + P digest 2079 ✖
24 pSB4C5 I 3896 ✖
25 pSB4C5 II 3896 ✖
26 pSB4C5 III 3896 ✖

R751 cells arrived. Made two plates without antibiotics.

Digestions were started:


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