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Team:Paris/Protocols transformation
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(New page: ==Protocol for bacterial transformation== * 250µl of DH5α competent bacteria (by RbCl) * Add 2µl of DNA (ex Biobrick) * Incubation during 20min on ice * Heat choc 45second at 42°C * ...) |
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==Protocol for bacterial transformation== | ==Protocol for bacterial transformation== | ||
Latest revision as of 15:58, 4 August 2009
Protocol for bacterial transformation
- 250µl of DH5α competent bacteria (by RbCl)
- Add 2µl of DNA (ex Biobrick)
- Incubation during 20min on ice
- Heat choc 45second at 42°C
- Leave on ice 2min
- Add 1ml of LB preheated at 42°C
- Incubation 1 hour at 37°C under agitation
- Put 100µl of culture under plate
- Centrifuge 2min at 6rpm MAX for 2 min
- Put 100µl of concentrated culture
- Incubate overnight at 37°C
